Rose E C, Jonas I E, Kappert H F
Poliklinik für Kieferothopädie, Albert-Ludwigs-Universität Freiburg, Germany.
J Orofac Orthop. 1998;59(5):253-64. doi: 10.1007/BF01321792.
The alloys used in orthodontics are subject in the moist environment of the oral cavity to various corrosion processes. If the products of the corrosion are introduced into a biological system they may cause changes. In the present investigation the corrosion rate of 23 different orthodontic wires (preformed arch wires and straight wires) made from 5 different alloys were examined in a nutrient medium by ICP-AES analysis, and the influence of the corrosion products on the cytotoxicity of a fibroblast culture was investigated using Mosmann's MTT test. The nickel-titanium wires Nitinol, Sentalloy and Original Chinese Wire and the beta-titanium alloy TMA had no effect on the rate of cell proliferation. Nor did stainless steel wires inhibit growth significantly, with the exception of Australian Wire and Wildcat Wire. The manganese-steel alloys Noninium h and Mezanium caused significant reductions in growth rate, which were attributed to the manganese ions released by the corrosion. The most severe growth inhibition was caused by the Co-Cr-Ni alloy Elgiloy, and this reaction is independent of the 4 levels of resilience. The degree of growth inhibition depended upon the concentration of corrosive cobalt and nickel ions in the eluate. In spite of the differences observed, all the orthodontic wires examined are graded under ISO-standard 10993-5 as "non-cytotoxic". The degree of toxicity was found to be determined essentially by the corrosion rate of the alloy and the cytotoxic characteristics of the resulting trace elements.
正畸中使用的合金在口腔潮湿环境中会经历各种腐蚀过程。如果腐蚀产物进入生物系统，它们可能会引起变化。在本研究中，通过电感耦合等离子体发射光谱分析法（ICP - AES）在营养培养基中检测了由5种不同合金制成的23种不同正畸钢丝（预成弓丝和直丝）的腐蚀速率，并使用莫斯曼MTT试验研究了腐蚀产物对成纤维细胞培养物细胞毒性的影响。镍钛丝Nitinol、Sentalloy和Original Chinese Wire以及β钛合金TMA对细胞增殖速率没有影响。除了澳大利亚钢丝和野猫钢丝外，不锈钢丝也没有显著抑制生长。锰钢合金Noninium h和Mezanium导致生长速率显著降低，这归因于腐蚀释放的锰离子。最严重的生长抑制是由钴 - 铬 - 镍合金Elgiloy引起的，并且这种反应与4种弹性水平无关。生长抑制程度取决于洗脱液中腐蚀性钴和镍离子的浓度。尽管观察到差异，但所有检测的正畸钢丝根据ISO标准10993 - 5都被评为“无细胞毒性”。发现毒性程度基本上由合金的腐蚀速率和所得微量元素的细胞毒性特征决定。
