一种用于探索人类针对刚地弓形虫细胞免疫的快速流式细胞术方法。
A rapid flow cytometric method to explore cellular immunity against Toxoplasma gondii in humans.
作者信息
Kahi S, Cozon G J, Greenland T, Wallon M, Gay-Andrieu F, Peyron F
机构信息
Université Claude Bernard Lyon I, JE 1947, Lyon, France.
出版信息
Clin Diagn Lab Immunol. 1998 Nov;5(6):745-8. doi: 10.1128/CDLI.5.6.745-748.1998.
Abstract
To assess cell-mediated immunity to Toxoplasma gondii, we evaluated the expression of the activation antigens CD69, CD71, and CD25 on T lymphocytes by flow cytometry after specific in vitro stimulation of whole blood from 127 T. gondii-positive and 63 T. gondii-negative patients. T lymphocytes from many seropositive individuals did not express CD69 at 24 h after T. gondii antigen stimulation, but CD71 and CD25 were easily detectable on T cells from seropositive individuals 7 days after specific activation. CD25 was mainly expressed by stimulated CD4(+) T cells, and its detection on total T cells was both a sensitive (98%) and a specific (97%) indicator of prior T. gondii infection. These results make flow cytometric detection of CD25 an excellent candidate for screening cell-mediated immunity to T. gondii in vitro and an interesting tool for the diagnosis of congenital infection.
摘要
为评估针对刚地弓形虫的细胞介导免疫，我们对127例弓形虫阳性患者和63例弓形虫阴性患者的全血进行特异性体外刺激后，通过流式细胞术评估了T淋巴细胞上活化抗原CD69、CD71和CD25的表达。许多血清阳性个体的T淋巴细胞在刚地弓形虫抗原刺激后24小时不表达CD69，但在特异性激活7天后，血清阳性个体的T细胞上可轻易检测到CD71和CD25。CD25主要由受刺激的CD4(+) T细胞表达，在总T细胞上检测到CD25是既往刚地弓形虫感染的敏感（98%）和特异（97%）指标。这些结果使流式细胞术检测CD25成为体外筛选针对刚地弓形虫的细胞介导免疫的极佳候选方法，也是诊断先天性感染的有趣工具。
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