Sironi M, Bandi C, Novati S, Scaglia M
Istituto di Patologia Generale Veterinaria, Università di Milano, Italy.
Parassitologia. 1997 Dec;39(4):437-9.
From small subunit ribosomal RNA gene sequences, a pair of PCR primers were designed to amplify a portion of this gene from five species of microsporidia. The amplified fragments encompass polymorphic restriction sites for the Hphl enzyme, resulting in different restriction patterns in the different species. We tested this identification method both on cultured microsporidia and on clinical samples. On cultured microsporidia the expected amplification bands were obtained even when DNA preparations from only ten spores were analysed. On clinical samples, identification of microsporidia was obtained from crude DNA preparations. This method allows for a rapid and easy diagnosis of human microsporidioses.
从小亚基核糖体RNA基因序列出发，设计了一对聚合酶链反应（PCR）引物，用于从五种微孢子虫中扩增该基因的一部分。扩增片段包含Hphl酶的多态性限制性位点，导致不同物种呈现不同的限制性图谱。我们在培养的微孢子虫和临床样本上都测试了这种鉴定方法。在培养的微孢子虫上，即使仅分析来自十个孢子的DNA制剂，也能获得预期的扩增条带。在临床样本上，从粗制DNA制剂中鉴定出了微孢子虫。该方法能够快速、简便地诊断人类微孢子虫病。
