Wang L S, Wu L H, Chang C J, Li W Y, Fahn H J, Huang M H, Chiu J H
Department of Surgery, Veterans' General Hospital, National Yang-Ming University, Taipei, Taiwan, Republic of China.
Scand Cardiovasc J. 1998;32(4):205-12. doi: 10.1080/14017439850139988.
The DNA content in oesophageal carcinoma and in sequential non-tumour mucosa was evaluated in 35 patients with oesophageal carcinoma, to explore the hypotheses that DNA distribution pattern and S-phase fraction can reflect malignant potential and that DNA aneuploidy can provide an early-warning signal of developing cancer. DNA flow cytometry was performed on 129 specimens from the tumours and on 119 specimens from non-tumour mucosa. Control specimens from gastric fundus had normal diploid DNA content and low S-phase fraction. Aneuploidy was found in 94.3% of the carcinoma specimens and intratumoral heterogeneity in 54.3%. Of the non-tumour specimens, 43.7% showed aneuploidy and none multiple aneuploidy. Pattern III distribution was present in 8.6% of the tumour specimens but not in non-tumour mucosa, where the incidence of aneuploidy rose with closeness to the tumour (p < 0.001). S-phase fraction was smaller in non-tumour than in tumour specimens (p < 0.0001). The study indicated that histologically tumour-free oesophageal mucosa may have a high malignant potential in patients with oesophageal carcinoma. The relative instability of such mucosa, with aneuploid cells and low S-phase fraction, may facilitate transition to abnormally proliferating cells in response to environmental signals. Cigarette smoking and alcohol may increase the risk of multicentric cancer development.
对35例食管癌患者的食管癌组织及连续的非肿瘤黏膜组织中的DNA含量进行了评估，以探讨DNA分布模式和S期分数能否反映恶性潜能，以及DNA非整倍体能否提供癌症发生的早期预警信号这两个假说。对129份肿瘤标本和119份非肿瘤黏膜标本进行了DNA流式细胞术检测。胃底对照标本的DNA含量为正常二倍体，S期分数较低。94.3%的癌组织标本存在非整倍体，54.3%存在肿瘤内异质性。在非肿瘤标本中，43.7%显示非整倍体，无一例为多重非整倍体。8.6%的肿瘤标本呈现III型分布，而非肿瘤黏膜中未出现，非肿瘤黏膜中非整倍体的发生率随与肿瘤距离的缩短而升高（p<0.001）。非肿瘤标本的S期分数低于肿瘤标本（p<0.0001）。该研究表明，在食管癌患者中，组织学上无肿瘤的食管黏膜可能具有较高的恶性潜能。这种黏膜相对不稳定，存在非整倍体细胞且S期分数较低，可能有助于其在环境信号作用下向异常增殖细胞转变。吸烟和饮酒可能增加多中心癌症发生的风险。
