联苯的真菌代谢
Fungal metabolism of biphenyl.
作者信息
Dodge R H, Cerniglia C E, Gibson D T
出版信息
Biochem J. 1979 Jan 15;178(1):223-30. doi: 10.1042/bj1780223.
Cunninghamella elegans grown on Sabouraud dextrose broth transformed biphenyl to produce 2-, 3- and 4-hydroxybiphenyl, as well as 4,4'-dihydroxybiphenyl as free phenols. A compound tentatively identified as 2,4'-dihydroxybiphenyl was also produced. When 4-hydroxybiphenyl or 2-hydroxybiphenyl replaced biphenyl as the substrate, C. elegans produced 4,4'-dihydroxybiphenyl and 2,5-dihydroxybiphenyl respectively. The compound identified as 2,4'-dihydroxybiphenyl was produced from both substrates. A survey of 11 species of fungi known to degrade hydrocarbons revealed two species that were comparable to C. elegans in their ability to convert biphenyl into free phenols. In addition to free phenolic metabolites, deconjugation experiments indicated that 44% of the known metabolites present in the culture filtrate were present in the form of conjugates. These results suggest that the transformation of biphenyl by C. elegans is similar to that found in mammalian systems.
在沙氏葡萄糖肉汤上生长的雅致小克银汉霉将联苯转化为2-、3-和4-羟基联苯，以及4,4'-二羟基联苯作为游离酚类。还产生了一种初步鉴定为2,4'-二羟基联苯的化合物。当4-羟基联苯或2-羟基联苯替代联苯作为底物时，雅致小克银汉霉分别产生4,4'-二羟基联苯和2,5-二羟基联苯。从这两种底物都产生了鉴定为2,4'-二羟基联苯的化合物。对已知能降解碳氢化合物的11种真菌进行的一项调查显示，有两种真菌在将联苯转化为游离酚类的能力方面与雅致小克银汉霉相当。除了游离酚类代谢产物外，去结合实验表明，培养滤液中存在的已知代谢产物有44%以结合物的形式存在。这些结果表明，雅致小克银汉霉对联苯的转化与在哺乳动物系统中发现的情况相似。
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