Günel E, Cağlayan F, Cağlayan O, Dilsiz A, Duman S, Aktan M
Department of Pediatric Surgery, Medical School of Selçuk University, Konya, Turkey.
J Pediatr Surg. 1998 Oct;33(10):1536-9. doi: 10.1016/s0022-3468(98)90492-4.
BACKGROUND/PURPOSE: A number of antioxidants are present in human tissues, which comprise the secondary defence system against the oxygen-free radicals and oxidative stress. Therefore, this study was undertaken in an animal model to investigate the effects of antioxidants such as vitamin E, vitamin C, methyl prednisolone, and mannitol, on the treatment of intestinal reperfusion injury when given during ischemia.
The study was performed on six groups of animals, each composed of six rabbits: Group I, mesenteric ischemia group; group R, ischemia-reperfusion group; group E, ischemia-reperfusion plus Vitamin E treatment; group C, ischemia-reperfusion plus vitamin C treatment; group CS, ischemia-reperfusion plus corticosteroid treatment; and Group M, ischemia-reperfusion plus mannitol treatment. Malondialdehyde (MDA) and glutathione (GSH) levels of the intestinal mucosa were assayed to reflect the free radical formation.
Mucosal injury scores in the M and C groups were significantly lower than the R group (P< .01 and P< .01, respectively), and in the E and CS groups, mucosal injury scores were not significantly different from R group. MDA levels in the M and C groups were significantly lower than the R group (P < .01 and P < .01, respectively). GSH levels in the E, C, and M groups, were significantly higher than R group (P < .01, P < .05, and P < .01, respectively).
The results of this study showed that antioxidant drugs, such as vitamin C and mannitol, may play a role in attenuating reperfusion injury of the gut demonstrated by depression of tissue MDA levels and by elevation of tissue GSH levels.
背景/目的：人体组织中存在多种抗氧化剂，它们构成了抵御氧自由基和氧化应激的二级防御系统。因此，本研究采用动物模型，探讨维生素E、维生素C、甲基泼尼松龙和甘露醇等抗氧化剂在缺血期间给予时对肠道再灌注损伤治疗的影响。
对六组动物进行研究，每组由六只兔子组成：第一组，肠系膜缺血组；R组，缺血-再灌注组；E组，缺血-再灌注加维生素E治疗组；C组，缺血-再灌注加维生素C治疗组；CS组，缺血-再灌注加皮质类固醇治疗组；M组，缺血-再灌注加甘露醇治疗组。检测肠黏膜丙二醛（MDA）和谷胱甘肽（GSH）水平，以反映自由基的形成。
M组和C组的黏膜损伤评分显著低于R组（分别为P<0.01和P<0.01），E组和CS组的黏膜损伤评分与R组无显著差异。M组和C组的MDA水平显著低于R组（分别为P<0.01和P<0.01）。E组、C组和M组的GSH水平显著高于R组（分别为P<0.01、P<0.05和P<0.01）。
本研究结果表明，抗氧化药物如维生素C和甘露醇，可能通过降低组织MDA水平和提高组织GSH水平，在减轻肠道再灌注损伤中发挥作用。
