Murata M, Inoue M, Arisue M, Kuboki Y, Nagai N
Second Department of Oral Surgery, School of Dentistry, Health Sciences University of Hokkaido, Tobetsu, Japan.
Int J Oral Maxillofac Surg. 1998 Oct;27(5):391-6. doi: 10.1016/s0901-5027(98)80071-4.
Partially purified bone morphogenetic protein (BMP) was delivered into two different types of carriers, porous particles of hydroxyapatite (PPHAP) and particles of insoluble bone matrix (IBM), and the ossification process was examined after subcutaneous implantation of the BMP/PPHAP and BMP/IBM in rats. The ossification in the BMP/PPHAP system was predominantly direct through bone formation similar to intramembranous ossification, whereas in the BMP/IBM system it was predominantly endochondral. The differences observed between the BMP/PPHAP and the BMP/IBM indicate the importance of the structure and nature of the carrier in the process of bone induction. The findings suggest that bone and cartilage differentiation is controlled not only by the regulation factor (BMP), but also by its interaction with the carrier, and that the BMP-induced cell differentiation is dependent upon the microenvironment derived from the carrier.
将部分纯化的骨形态发生蛋白（BMP）植入两种不同类型的载体中，即羟基磷灰石多孔颗粒（PPHAP）和不溶性骨基质颗粒（IBM），在大鼠皮下植入BMP/PPHAP和BMP/IBM后检查骨化过程。BMP/PPHAP系统中的骨化主要是通过类似于膜内骨化的直接骨形成，而在BMP/IBM系统中则主要是软骨内骨化。BMP/PPHAP和BMP/IBM之间观察到的差异表明载体的结构和性质在骨诱导过程中的重要性。这些发现表明，骨和软骨分化不仅受调节因子（BMP）的控制，还受其与载体相互作用的控制，并且BMP诱导的细胞分化取决于载体衍生的微环境。
