Díaz Torres H, Silva Cabrera E, Rodríguez García O, Bárcenas Moses J, Lubián Caballero A L
Laboratorio de Investigaciones del SIDA, San José de las Lajas, provincia de La Habana, Cuba.
Rev Cubana Med Trop. 1996;48(3):188-91.
The presence of antibodies against the HIV protein of 24 kd was studies by the parallel use of the DAVIH BLOT western blot and of the DAVIH AC P24 ELISA in serum samples from 176 patients at different HIV-1 infection stages. The results were correlated with the clinical classification of the patient at the moment of taking the sample and with the further evolution during 6 months. 57% of the patients with opportunistic minor infections and 96% of AIDS patients had low antibodies titres. Dead patients showed no reactivity or presented very low titres in samples taken before dying. Different titrations were observed in serum groups with an apparently uniform reactivity in the western blot. The results show and adequate clinical and serological correlation. Therefore, the DAVIH AC P24 ELISA could be useful in the clinical follow-up of HIV-1 infected persons.
通过平行使用DAVIH印迹法（蛋白质免疫印迹法）和DAVIH AC P24酶联免疫吸附测定法，对176例处于不同HIV-1感染阶段患者的血清样本中针对24kd HIV蛋白的抗体存在情况进行了研究。研究结果与采样时患者的临床分类以及随后6个月内的病情进展相关。57%患有轻度机会性感染的患者和96%的艾滋病患者抗体滴度较低。死亡患者在死前采集的样本中无反应性或抗体滴度极低。在蛋白质免疫印迹法中反应性明显一致的血清组中观察到了不同的滴定度。结果显示出临床与血清学之间的充分相关性。因此，DAVIH AC P24酶联免疫吸附测定法可用于HIV-1感染者的临床随访。
