用一种新型聚合酶链反应检测法对乙型肝炎病毒DNA进行定量检测。
Quantitative detection of hepatitis B virus DNA with a new PCR assay.
作者信息
Kessler H H, Pierer K, Santner B I, Vellimedu S K, Stelzl E, Lackner H, Moser A, Marth E
机构信息
Institute of Hygiene, Karl-Franzens-University, Graz, Austria.
出版信息
Clin Chem Lab Med. 1998 Aug;36(8):601-4. doi: 10.1515/CCLM.1998.105.
The Amplicor HBV Monitor Test for quantitative detection of serum hepatitis B virus (HBV) DNA has recently been introduced. This assay is based on PCR and a non-radioactive hybridization and detection system on microwell plates. Evaluation in a routine diagnostic laboratory showed excellent sensitivity and adequate reproducibility; however, a more automated format would be desirable. The Amplicor HBV Monitor Test is useful for recognizing those patients who might benefit from antiviral treatment and for evaluation of the efficacy of anti-hepatitis B virus treatment.
用于定量检测血清乙型肝炎病毒（HBV）DNA的Amplicor HBV监测检测法最近已被引入。该检测基于聚合酶链反应（PCR）以及微孔板上的非放射性杂交和检测系统。在常规诊断实验室中的评估显示出极佳的灵敏度和足够的可重复性；然而，更自动化的形式会更理想。Amplicor HBV监测检测法对于识别那些可能从抗病毒治疗中获益的患者以及评估抗乙型肝炎病毒治疗的疗效很有用。
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