半自动COBAS AMPLICOR乙型肝炎病毒（HBV）监测检测与基于微孔板的手动AMPLICOR HBV监测检测的比较评估
Comparative evaluation of semiautomated COBAS AMPLICOR hepatitis B virus (HBV) monitor test and manual microwell plate-based AMPLICOR HBV MONITOR test.
作者信息
Marin I J, Poljak M, Seme K, Meglic-Volkar J, Maticic M, Lesnicar G, Brinovec V
机构信息
Institute of Microbiology and Immunology, Medical Faculty, Ljubljana, Slovenia.
出版信息
J Clin Microbiol. 2001 Feb;39(2):758-61. doi: 10.1128/JCM.39.2.758-761.2001.
Abstract
Comparative evaluation of the semiautomated COBAS AMPLICOR hepatitis B virus (HBV) MONITOR Test (COBAS-HBV) and manual AMPLICOR HBV MONITOR Test (AMPLICOR-HBV) on 208 serum samples revealed no significant difference in the sensitivities of the two assays. Twenty samples tested HBV DNA negative and 183 samples tested HBV DNA positive by both assays. Three samples tested positive by COBAS-HBV only and two samples tested positive by AMPLICOR-HBV only. HBV DNA concentrations determined by the two assays were significantly related (n = 183, r = 0.97, P < 0.0001), which indicates that COBAS-HBV could replace AMPLICOR-HBV. The major inconvenience of COBAS-HBV is the required performance of appropriate predilutions of high-titer samples in order to extend the narrow dynamic range of the assay.
摘要
对208份血清样本进行的半自动COBAS AMPLICOR乙型肝炎病毒（HBV）监测试验（COBAS-HBV）和手工AMPLICOR HBV监测试验（AMPLICOR-HBV）的比较评估显示，两种检测方法的灵敏度无显著差异。两种检测方法均显示20份样本HBV DNA阴性，183份样本HBV DNA阳性。3份样本仅COBAS-HBV检测呈阳性，2份样本仅AMPLICOR-HBV检测呈阳性。两种检测方法测定的HBV DNA浓度显著相关（n = 183，r = 0.97，P < 0.0001），这表明COBAS-HBV可替代AMPLICOR-HBV。COBAS-HBV的主要不便之处在于，为了扩展该检测方法狭窄的动态范围，需要对高滴度样本进行适当的预稀释。
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