Human leukocyte antigen class II genotype in patients with recurrent fetal miscarriage who are positive for anticardiolipin antibody.

OBJECTIVE

To elucidate the relationship between human leukocyte antigen (HLA) class II genotypes and patients with recurrent fetal miscarriage who are positive for anticardiolipin antibody.

DESIGN

Prospective clinical study.

SETTING

Institutional practice at the outpatient clinic for infertility, Niigata University Medical Hospital.

PATIENT(S): Patients with recurrent fetal miscarriage who were positive for anticardiolipin antibody and normal fertile women.

INTERVENTION(S): Genomic DNA was extracted from peripheral mononuclear cells.

MAIN OUTCOME MEASURE(S): Human leukocyte antigen class II genotype was determined using a polymerase chain reaction restriction fragment length polymorphism method.

RESULT(S): The frequencies of DRB10403 and DRB10410 were significantly higher in the patient group than in the control group. The frequency of DRB104 also was significantly higher in the patient group. As for HLA-DQ genotype, the frequency of HLA-DQB10501 was significantly lower in the patient group.

CONCLUSION

Human leukocyte antigen systems appear to be involved in the genesis of antiphospholipid syndrome.