Takakuwa Koichi, Honda Keisuke, Yokoo Tomokazu, Hataya Isao, Tamura Masaki, Tanaka Kenichi
Department of Obstetrics and Gynecology, Niigata University School of Medicine, 1-757, Asahimachi-dori, Niigata 951-8510, Japan.
Clin Immunol. 2006 Jan;118(1):101-7. doi: 10.1016/j.clim.2005.07.011. Epub 2005 Aug 24.
It is hypothesized that patients having unexplained recurrent miscarriage lack an appropriate immune reaction against their partner's antigens, which means possible compatibility of HLA antigens between the patient couples. The conclusion, however, has not yet been achieved, so the purpose was to determine whether significant compatibility of HLA class II exists between the couples. The HLA-DRB1 and -DQB1 genotypes were determined using PCR-RFLP method in 91 patient couples and in 72 normal couples. The number of patient couples with zero-allele mismatch was not significantly different compared with that of control couples regarding HLA-DRB1 genotype and phenotype, as well as regarding HLA-DQB1 genotype and phenotype. While the number of patient couples with zero- and one-allele mismatch was significantly higher compared with that in control as to HLA-DR and -DQ phenotype (P=0.029 by Chi-square test). In conclusion, it is suggested that the compatibility of HLA class II antigens between couples might be involved in the genesis of unexplained recurrent miscarriage.
据推测，患有不明原因复发性流产的患者缺乏针对其伴侣抗原的适当免疫反应，这意味着患者夫妇之间可能存在HLA抗原相容性。然而，尚未得出这一结论，因此目的是确定夫妇之间是否存在显著的HLA II类相容性。采用PCR-RFLP方法对91对患者夫妇和72对正常夫妇的HLA-DRB1和-DQB1基因型进行了测定。在HLA-DRB1基因型和表型以及HLA-DQB1基因型和表型方面，零等位基因错配的患者夫妇数量与对照夫妇相比无显著差异。而在HLA-DR和-DQ表型方面，零等位基因和一等位基因错配的患者夫妇数量显著高于对照组（卡方检验P=0.029）。总之，提示夫妇之间HLA II类抗原的相容性可能与不明原因复发性流产的发生有关。
