Wallström E, Khademi M, Andersson M, Weissert R, Linington C, Olsson T
Neuroimmunology Unit, Department of Medicine, Karolinska Institute, Stockholm, Sweden.
Eur J Immunol. 1998 Oct;28(10):3329-35. doi: 10.1002/(SICI)1521-4141(199810)28:10<3329::AID-IMMU3329>3.0.CO;2-B.
Multiple sclerosis (MS) is a central nervous system-specific inflammatory and demyelinating disease where a myelin-directed autoimmune response is thought to be pathogenetically relevant. Myelin oligodendrocyte glycoprotein (MOG) is a surface-exposed minor myelin component that is a prime candidate autoantigen. We have investigated peripheral blood lymphocyte responses to synthetic 15-26 amino acids long overlapping MOG peptides in 20 MS patients and 14 healthy controls with the MS-associated HLA haplotype DR2(15). There were significantly increased responses, in terms of numbers of cells secreting IFN-gamma detected by Elispot in response to several MOG-derived peptides in the MS patients, but not the healthy controls. MOG peptide 63-87 evoked the strongest response, and the stimulatory property of this peptide was confirmed in additional DR2(15)+ MS patients where a peptide concentration-dependent proliferative response, which was inhibited by the addition of anti-HLA class II antibodies, was observed. This is the first work detailing putative immunodominant T cell epitopes of MOG in DR2(15)+ MS patients.
多发性硬化症（MS）是一种中枢神经系统特异性炎症性脱髓鞘疾病，其中针对髓鞘的自身免疫反应被认为与发病机制相关。髓鞘少突胶质细胞糖蛋白（MOG）是一种暴露于表面的次要髓鞘成分，是主要的自身抗原候选物。我们研究了20例MS患者和14例具有MS相关HLA单倍型DR2(15)的健康对照者外周血淋巴细胞对合成的15 - 26个氨基酸长的重叠MOG肽的反应。通过酶联免疫斑点法检测，MS患者对几种MOG衍生肽产生分泌γ干扰素的细胞数量反应显著增加，而健康对照者则无此现象。MOG肽63 - 87引发的反应最强，在另外的DR2(15)+ MS患者中证实了该肽的刺激特性，观察到其呈肽浓度依赖性增殖反应，且添加抗HLA II类抗体可抑制该反应。这是第一项详细描述DR2(15)+ MS患者中MOG假定免疫显性T细胞表位的研究。
