Vine W, Blase E, Koda J, Young A
Amylin Pharmaceuticals Inc., San Diego, CA 92121, USA.
Horm Metab Res. 1998 Sep;30(9):581-5. doi: 10.1055/s-2007-978937.
Amylin is a 37-amino acid peptide co-secreted from the pancreatic beta-cell with insulin in response to nutrient stimuli. Plasma amylin concentrations in the rat are reported to vary widely. We have employed a recently-developed immunoenzymometric assay to quantify plasma amylin concentrations in fasted, fed and glucose-administered rats. Fasted amylin concentrations ranged between 1.02+/-0.09 and 1.63+/-0.15pM among three different common rat strains, and increased up to 7.70+/-0.80 pM after feeding. The differences among strains and between fasted and fed rats were all significant at P<0.01 or less. Intravenous glucose administration (5.2 mmol/kg) also significantly increased plasma amylin concentrations in fasted rats from 1.5+/-0.3pM to 3.4+/-0.5pM, and in fed rats from 4.6+/-1.1 pM to 9.1+/-1.7 pM. Plasma amylin/insulin molar ratios ranged between 2.3+/-0.2% and 3.6+/-0.5% (mean 3.0%), but did not differ among strains, or between the fasted vs fed state in any strain. In conclusion, a new sensitive immunoenzymometric assay revealed fasting plasma concentrations which are lower than previously reported, and which are significantly increased by stimulation with feeding or glucose administration.
胰淀素是一种由胰腺β细胞与胰岛素共同分泌的含37个氨基酸的肽，其分泌受营养刺激的影响。据报道，大鼠血浆中的胰淀素浓度差异很大。我们采用了一种最近开发的免疫酶联测定法来定量测定禁食、进食和注射葡萄糖的大鼠血浆中的胰淀素浓度。在三种不同的常见大鼠品系中，禁食状态下的胰淀素浓度在1.02±0.09至1.63±0.15皮摩尔之间，进食后可升至7.70±0.80皮摩尔。品系之间以及禁食和进食大鼠之间的差异在P<0.01或更低时均具有显著性。静脉注射葡萄糖（5.2毫摩尔/千克）也显著提高了禁食大鼠的血浆胰淀素浓度，从1.5±0.3皮摩尔升至3.4±0.5皮摩尔，进食大鼠的血浆胰淀素浓度从4.6±1.1皮摩尔升至9.1±1.7皮摩尔。血浆胰淀素/胰岛素摩尔比在2.3±0.2%至3.6±0.5%之间（平均3.0%），但在品系之间或任何品系的禁食与进食状态之间均无差异。总之，一种新的灵敏免疫酶联测定法显示，禁食时的血浆浓度低于先前报道的水平，并且通过进食或注射葡萄糖刺激后显著升高。
