Yeum K J, Ahn S H, Rupp de Paiva S A, Lee-Kim Y C, Krinsky N I, Russell R M
Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, Boston, MA, USA.
J Nutr. 1998 Nov;128(11):1920-6. doi: 10.1093/jn/128.11.1920.
To evaluate the relationship between carotenoid concentrations in serum and breast tissue, we measured serum carotenoid concentrations and endogenous carotenoid levels in breast adipose tissue of women with benign breast tumor (n = 46) or breast cancer (n = 44). Before extraction, serum was digested with lipase and cholesterol esterase, and breast adipose tissue was saponified. Serum and tissue carotenoids were extracted with ether/hexane and measured by using HPLC with a C30 column. Serum retinoic acid was extracted with chloroform/methanol and measured using HPLC with a C18 column. There were no significant differences in serum carotenoids [lutein, zeaxanthin, cryptoxanthin (both alpha- and beta-), alpha-carotene, all-trans beta-carotene, 13-cis beta-carotene and lycopene], retinoids (retinol, all-trans and 13-cis retinoic acids), and alpha- and gamma- tocopherol concentrations between benign breast tumor patients and breast cancer patients. A substantial amount of 9-cis beta-carotene was present in adipose tissue and was the only carotenoid that had a significantly lower level in benign breast tumor patients than in breast cancer patients. Correlations between carotenoid concentrations in serum and in breast adipose tissue were determined by combining the data of the two groups. Concentrations of the major serum carotenoids except cryptoxanthin showed significant correlations with breast adipose tissue carotenoid levels. When the concentrations of serum carotenoids were adjusted for serum triglycerides or LDL, correlations between serum carotenoid concentrations and breast adipose tissue carotenoid levels markedly increased, including that of cryptoxanthin (P <0. 001). The strong correlation between serum carotenoid concentrations and endogenous breast adipose tissue carotenoid levels indicate that dietary intake influences adipose tissue carotenoid levels as well as serum concentrations, and that adipose tissue is a dynamic reservoir of fat-soluble nutrients.
为评估血清和乳腺组织中类胡萝卜素浓度之间的关系，我们测量了患有良性乳腺肿瘤（n = 46）或乳腺癌（n = 44）的女性血清类胡萝卜素浓度以及乳腺脂肪组织中的内源性类胡萝卜素水平。提取前，血清用脂肪酶和胆固醇酯酶消化，乳腺脂肪组织进行皂化处理。血清和组织类胡萝卜素用乙醚/己烷提取，并用C30柱的高效液相色谱法进行测量。血清视黄酸用氯仿/甲醇提取，并用C18柱的高效液相色谱法进行测量。良性乳腺肿瘤患者和乳腺癌患者之间的血清类胡萝卜素（叶黄素、玉米黄质、隐黄质（α和β）、α-胡萝卜素、全反式β-胡萝卜素、13-顺式β-胡萝卜素和番茄红素）、类视黄醇（视黄醇、全反式和13-顺式视黄酸）以及α和γ-生育酚浓度没有显著差异。脂肪组织中存在大量的9-顺式β-胡萝卜素，它是唯一在良性乳腺肿瘤患者中水平显著低于乳腺癌患者的类胡萝卜素。通过合并两组数据确定血清和乳腺脂肪组织中类胡萝卜素浓度之间的相关性。除隐黄质外，主要血清类胡萝卜素的浓度与乳腺脂肪组织类胡萝卜素水平呈显著相关。当血清类胡萝卜素浓度根据血清甘油三酯或低密度脂蛋白进行调整时，血清类胡萝卜素浓度与乳腺脂肪组织类胡萝卜素水平之间的相关性显著增加，包括隐黄质（P <0.001）。血清类胡萝卜素浓度与乳腺脂肪组织内源性类胡萝卜素水平之间的强相关性表明，饮食摄入会影响脂肪组织类胡萝卜素水平以及血清浓度，并且脂肪组织是脂溶性营养素的动态储存库。
