硫氧还蛋白超家族的一个新型核成员。
A novel nuclear member of the thioredoxin superfamily.
作者信息
Laughner B J, Sehnke P C, Ferl R J
机构信息
Plant Molecular and Cellular Biology Program, Department of Horticultural Sciences, University of Florida, Gainesville, Florida 32611, USA.
出版信息
Plant Physiol. 1998 Nov;118(3):987-96. doi: 10.1104/pp.118.3.987.
Abstract
We describe the isolation and characterization of a cDNA encoding maize (Zea mays L.) nucleoredoxin (NRX), a novel nuclear protein that is a member of the thioredoxin (TRX) superfamily. NRX is composed of three TRX-like modules arranged as direct repeats of the classic TRX domain. The first and third modules contain the amino acid sequence WCPPC, which indicates the potential for TRX oxidoreductase activity, and insulin reduction assays indicate that at least the third module possesses TRX enzymatic activity. The carboxy terminus of NRX is a non-TRX module that possesses C residues in the proper sequence context to form a Zn finger. Immunolocalization preferentially to the nucleus within developing maize kernels suggests a potential for directed alteration of the reduction state of transcription factors as part of the events and pathways that regulate gene transcription.
摘要
我们描述了一种编码玉米（Zea mays L.）核硫氧还蛋白（NRX）的cDNA的分离和特性，NRX是一种新型核蛋白，属于硫氧还蛋白（TRX）超家族成员。NRX由三个类似TRX的模块组成，这些模块排列成经典TRX结构域的直接重复序列。第一个和第三个模块包含氨基酸序列WCPPC，这表明具有TRX氧化还原酶活性的潜力，胰岛素还原试验表明至少第三个模块具有TRX酶活性。NRX的羧基末端是一个非TRX模块，其具有以适当序列形成锌指的C残基。在发育中的玉米粒中优先定位于细胞核的免疫定位表明，作为调节基因转录的事件和途径的一部分，转录因子还原状态的定向改变具有可能性。
相似文献
1
A novel nuclear member of the thioredoxin superfamily.硫氧还蛋白超家族的一个新型核成员。
Plant Physiol. 1998 Nov;118(3):987-96. doi: 10.1104/pp.118.3.987.
2
Cloning and characterization of the nucleoredoxin gene that encodes a novel nuclear protein related to thioredoxin.
Genomics. 1997 Feb 1;39(3):331-9. doi: 10.1006/geno.1996.4493.
3
Nucleoredoxin, a novel thioredoxin family member involved in cell growth and differentiation.核氧化还原蛋白，一种参与细胞生长和分化的新型硫氧还蛋白家族成员。
Antioxid Redox Signal. 2007 Aug;9(8):1035-57. doi: 10.1089/ars.2007.1550.
4
NTR/NRX define a new thioredoxin system in the nucleus of Arabidopsis thaliana cells.NTR/NRX 在拟南芥细胞核中定义了一个新的硫氧还蛋白系统。
Mol Plant. 2014 Jan;7(1):30-44. doi: 10.1093/mp/sst162. Epub 2013 Nov 19.
5
Isolation of a gene encoding a glycosylated cytokinin oxidase from maize.从玉米中分离出一个编码糖基化细胞分裂素氧化酶的基因。
Biochem Biophys Res Commun. 1999 Feb 16;255(2):328-33. doi: 10.1006/bbrc.1999.0199.
6
Cloning and sequencing of a corn (Zea mays) nuclear gene coding for the chloroplast specific catalytic subunit of ferredoxin-thioredoxin reductase.
Biochim Biophys Acta. 1993 Nov 2;1183(1):207-9. doi: 10.1016/0005-2728(93)90020-g.
7
Expression of maize gamma zein C-terminus in Escherichia coli.玉米γ-醇溶蛋白C末端在大肠杆菌中的表达
Protein Expr Purif. 1998 Jun;13(1):1-8. doi: 10.1006/prep.1998.0858.
8
The maize Single myb histone 1 gene, Smh1, belongs to a novel gene family and encodes a protein that binds telomere DNA repeats in vitro.玉米单myb组蛋白1基因（Smh1）属于一个新的基因家族，编码一种在体外能结合端粒DNA重复序列的蛋白质。
Plant Physiol. 2003 Nov;133(3):1336-50. doi: 10.1104/pp.103.026856. Epub 2003 Oct 23.
9
Phylogenetic analyses identify 10 classes of the protein disulfide isomerase family in plants, including single-domain protein disulfide isomerase-related proteins.系统发育分析确定了植物中蛋白质二硫键异构酶家族的10个类别，包括单结构域蛋白质二硫键异构酶相关蛋白。
Plant Physiol. 2005 Feb;137(2):762-78. doi: 10.1104/pp.104.056507. Epub 2005 Jan 28.
10
Cytokinin oxidase from Zea mays: purification, cDNA cloning and expression in moss protoplasts.玉米细胞分裂素氧化酶：纯化、cDNA克隆及在苔藓原生质体中的表达
Plant J. 1999 Mar;17(6):615-26. doi: 10.1046/j.1365-313x.1999.00408.x.
引用本文的文献
1
Genome-wide identification of the nuclear redox protein gene family revealed its potential role in drought stress tolerance in rice.全基因组范围内对核氧化还原蛋白基因家族的鉴定揭示了其在水稻耐旱胁迫中的潜在作用。
Front Plant Sci. 2025 Apr 22;16:1562718. doi: 10.3389/fpls.2025.1562718. eCollection 2025.
2
Genome-wide identification, characterization, evolution, and expression pattern analyses of the typical thioredoxin gene family in wheat ( L.).小麦（L.）中典型硫氧还蛋白基因家族的全基因组鉴定、特征分析、进化及表达模式分析
Front Plant Sci. 2022 Dec 22;13:1020584. doi: 10.3389/fpls.2022.1020584. eCollection 2022.
3
Genome-wide identification of foxtail millet's TRX family and a functional analysis of in response to drought and salt stresses in transgenic .谷子TRX家族的全基因组鉴定及转基因植株中其对干旱和盐胁迫响应的功能分析
Front Plant Sci. 2022 Sep 26;13:946037. doi: 10.3389/fpls.2022.946037. eCollection 2022.
4
Nucleoredoxin Plays a Key Role in the Maintenance of Retinal Pigmented Epithelium Differentiation.核氧化还原蛋白在维持视网膜色素上皮细胞分化中起关键作用。
Antioxidants (Basel). 2022 Jun 1;11(6):1106. doi: 10.3390/antiox11061106.
5
Nucleoredoxin Gene Positively Regulates Drought Tolerance in Transgenic Wheat ( L.).核氧化还原蛋白基因正向调控转基因小麦（L.）的耐旱性。
Front Plant Sci. 2021 Nov 11;12:756338. doi: 10.3389/fpls.2021.756338. eCollection 2021.
6
Identification of a 193 bp promoter region of TaNRX1-D gene from common wheat that contributes to osmotic or ABA stress inducibility in transgenic Arabidopsis.从普通小麦中鉴定出 TaNRX1-D 基因的 193bp 启动子区域，该区域有助于转基因拟南芥对渗透或 ABA 胁迫的诱导。
Genes Genomics. 2021 Sep;43(9):1035-1048. doi: 10.1007/s13258-021-01115-x. Epub 2021 Jun 18.
7
Redox-Dependent Structural Modification of Nucleoredoxin Triggers Defense Responses against in .核氧化还原蛋白的氧化还原依赖性结构修饰触发了对植物中（原文此处in后缺少具体内容）的防御反应。
Int J Mol Sci. 2020 Dec 2;21(23):9196. doi: 10.3390/ijms21239196.
8
Comparative protein profiles of Butea superba tubers under seasonal changes.季节变化下紫铆块茎的蛋白质图谱比较
Mol Biol Rep. 2016 Jul;43(7):719-36. doi: 10.1007/s11033-016-4010-2. Epub 2016 May 19.
9
Comparative Analysis of Sorghum bicolor Proteome in Response to Drought Stress and following Recovery.高粱响应干旱胁迫及恢复后的蛋白质组比较分析
Int J Proteomics. 2014;2014:395905. doi: 10.1155/2014/395905. Epub 2014 Oct 1.
10
Systems Analysis of the Response of Photosynthesis, Metabolism, and Growth to an Increase in Irradiance in the Photosynthetic Model Organism Chlamydomonas reinhardtii.光合模式生物莱茵衣藻中光合作用、代谢和生长对光照增加的响应的系统分析。
Plant Cell. 2014 Jun;26(6):2310-2350. doi: 10.1105/tpc.114.124537. Epub 2014 Jun 3.
本文引用的文献
1
ARF-B(2): A Protein Complex that Specifically Binds to Part of the Anaerobic Response Element of Maize Adh 1.ARF-B(2)：一种特异性结合玉米醇脱氢酶1（Maize Adh 1）厌氧反应元件部分区域的蛋白质复合物。
Plant Physiol. 1990 Jul;93(3):1094-101. doi: 10.1104/pp.93.3.1094.
2
The Miniature1 Seed Locus of Maize Encodes a Cell Wall Invertase Required for Normal Development of Endosperm and Maternal Cells in the Pedicel.玉米的微型1种子基因座编码一种细胞壁转化酶，该酶是胚乳和花梗中母细胞正常发育所必需的。
Plant Cell. 1996 Jun;8(6):971-983. doi: 10.1105/tpc.8.6.971.
3
Cloning, expression, and characterization of a novel Escherichia coli thioredoxin.一种新型大肠杆菌硫氧还蛋白的克隆、表达及特性分析
J Biol Chem. 1997 Dec 5;272(49):30841-7. doi: 10.1074/jbc.272.49.30841.
4
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.空位BLAST和位置特异性迭代BLAST：新一代蛋白质数据库搜索程序。
Nucleic Acids Res. 1997 Sep 1;25(17):3389-402. doi: 10.1093/nar/25.17.3389.
5
Redox regulation of cellular activation.细胞活化的氧化还原调节
Annu Rev Immunol. 1997;15:351-69. doi: 10.1146/annurev.immunol.15.1.351.
6
Cloning and characterization of the nucleoredoxin gene that encodes a novel nuclear protein related to thioredoxin.
Genomics. 1997 Feb 1;39(3):331-9. doi: 10.1006/geno.1996.4493.
7
AP-1 transcriptional activity is regulated by a direct association between thioredoxin and Ref-1.AP-1转录活性受硫氧还蛋白与Ref-1之间的直接关联调控。
Proc Natl Acad Sci U S A. 1997 Apr 15;94(8):3633-8. doi: 10.1073/pnas.94.8.3633.
8
The folding catalyst protein disulfide isomerase is constructed of active and inactive thioredoxin modules.折叠催化剂蛋白二硫键异构酶由活性和非活性硫氧还蛋白模块构成。
Curr Biol. 1997 Apr 1;7(4):239-45. doi: 10.1016/s0960-9822(06)00119-9.
9
Cloning and expression of a novel mammalian thioredoxin.一种新型哺乳动物硫氧还蛋白的克隆与表达
J Biol Chem. 1997 Jan 31;272(5):2936-41. doi: 10.1074/jbc.272.5.2936.
10
Joining of DNA fragments by repeated cycles of denaturation, annealing and extension.
Biotechniques. 1996 Jun;20(6):954-5. doi: 10.2144/96206bm01.
Zotero 插件