高粱响应干旱胁迫及恢复后的蛋白质组比较分析
Comparative Analysis of Sorghum bicolor Proteome in Response to Drought Stress and following Recovery.
作者信息
Jedmowski Christoph, Ashoub Ahmed, Beckhaus Tobias, Berberich Thomas, Karas Michael, Brüggemann Wolfgang
机构信息
Institute of Ecology, Evolution, and Diversity, Johann Wolfgang Goethe-University Frankfurt, Max-Von-Laue Straße 13, 60438 Frankfurt, Germany.
Institute of Ecology, Evolution, and Diversity, Johann Wolfgang Goethe-University Frankfurt, Max-Von-Laue Straße 13, 60438 Frankfurt, Germany ; Biodiversity and Climate Research Centre (BiK-F), 60325, Frankfurt am Main, Germany ; Agricultural Genetic Engineering Research Institute (AGERI), ARC, Giza 12619, Egypt.
出版信息
Int J Proteomics. 2014;2014:395905. doi: 10.1155/2014/395905. Epub 2014 Oct 1.
The adaptive response of Sorghum bicolor landraces from Egypt to drought stress and following recovery was analyzed using two-dimensional difference gel electrophoresis, 2D-DIGE. Physiological measurements and proteome alterations of accession number 11434, drought tolerant, and accession number 11431, drought sensitive, were compared to their relative control values after drought stress and following recovery. Differentially expressed proteins were analysed by Matrix assisted laser desorption ionisation time-of-flight mass spectrometry, MALDI-TOF-MS. Alterations in protein contents related to the energy balance, metabolism (sensu Mewes et al. 1997), and chaperons were the most apparent features to elucidate the differences between the drought tolerant and sensitive accessions. Further alterations in the levels of proteins related to transcription and protein synthesis are discussed.
利用二维差异凝胶电泳（2D-DIGE）分析了来自埃及的双色高粱地方品种对干旱胁迫及后续恢复的适应性反应。将耐旱的编号11434和干旱敏感的编号11431在干旱胁迫后及恢复后的生理测量值和蛋白质组变化与其相对对照值进行了比较。通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）分析差异表达蛋白。与能量平衡、代谢（如Mewes等人，1997年所述）和伴侣蛋白相关的蛋白质含量变化是阐明耐旱和敏感品种之间差异的最明显特征。还讨论了与转录和蛋白质合成相关的蛋白质水平的进一步变化。
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