Card J P
Department of Neuroscience, University of Pittsburgh, PA 15217, USA.
Neurosci Biobehav Rev. 1998 Oct;22(6):685-94. doi: 10.1016/s0149-7634(98)00007-4.
The development of neurotrophic alpha herpesviruses for transneuronal analysis of neuronal circuitry has emerged from interdisciplinary characterizations of the viral life cycle and the defense response mounted by the nervous system to contain and eliminate the infection. Important findings from a number of fields have combined to provide compelling evidence that these viruses, when used appropriately, are powerful probes of multisynaptic circuits. These studies have also revealed that a number of variables can influence the outcome of infection and should be considered in designing and interpreting data derived from studies employing this experimental approach. The purpose of this paper is to review the literature that has established this experimental approach as a viable method for transynaptic analysis of neuronal circuitry and to define the factors that should be considered in applying this technology.
用于神经元回路跨神经元分析的神经营养性甲型疱疹病毒的开发，源于对病毒生命周期以及神经系统为控制和消除感染而产生的防御反应的跨学科表征。多个领域的重要发现相结合，提供了令人信服的证据，表明这些病毒在适当使用时，是多突触回路的有力探针。这些研究还表明，许多变量会影响感染结果，在设计和解释采用这种实验方法得出的数据时应予以考虑。本文的目的是回顾将这种实验方法确立为神经元回路跨突触分析可行方法的文献，并确定应用该技术时应考虑的因素。
