Norgren R B, Lehman M N
Department of Cell Biology and Anatomy, University of Nebraska Medical Center, Omaha 68198, USA.
Neurosci Biobehav Rev. 1998 Oct;22(6):695-708. doi: 10.1016/s0149-7634(98)00008-6.
Determining the connections of neural systems is critical for determining how they function. In this review, we focus on the use of HSV-1 and HSV-2 as transneuronal tracers. Using HSV to examine neural circuits is technically simple. HSV is injected into the area of interest, and after several days, the animals are perfused and processed for immunohistochemistry with antibodies to HSV proteins. Variables which influence HSV infection include species of host, age of host, titre of virus, strain of virus and phenotype of infected cell. The choice of strain of HSV is critically important. Several strains of HSV-1 and HSV-2 have been utilized for purposes of transneuronal tract-tracing. HSV has been used successfully to study neuronal circuitry in a variety of different neuroanatomical systems including the somatosensory, olfactory, visual, motor, autonomic and limbic systems.
确定神经系统的连接对于了解其功能至关重要。在本综述中，我们重点关注单纯疱疹病毒1型（HSV-1）和单纯疱疹病毒2型（HSV-2）作为跨神经元示踪剂的应用。使用HSV检测神经回路在技术上很简单。将HSV注入感兴趣的区域，几天后，对动物进行灌注并用针对HSV蛋白的抗体进行免疫组织化学处理。影响HSV感染的变量包括宿主物种、宿主年龄、病毒滴度、病毒株和被感染细胞的表型。HSV毒株的选择至关重要。HSV-1和HSV-2的几种毒株已被用于跨神经元通路追踪。HSV已成功用于研究多种不同神经解剖系统中的神经元回路，包括躯体感觉、嗅觉、视觉、运动、自主和边缘系统。
