Chakrabarti M K, Bhattacharya J, Sinha A K, De S P
National Institute of Cholera and Enteric Diseases, Beliaghata, Calcutta, India.
Zentralbl Bakteriol. 1995 Oct;282(4):436-41. doi: 10.1016/s0934-8840(11)80716-4.
Antiserum against outer membrane preparation of a Kanagawa phenomenon-positive strain of Vibrio parahaemolyticus were raised in rabbits and absorbed with their lipopolysaccharide. The anti-outer membrane protein serum and its Fab (IgG) fragment inhibited the adherence of Kanagawa-positive strains to rabbit intestinal epithelial cells in vitro. Preincubation of rabbit intestinal epithelial cells with outer membrane preparation also inhibited the adherence of these bacteria. Anti-lipopolysaccharide serum or its Fab (IgG) fragment did not inhibit adherence of V. parahaemolyticus. Moreover, pre-treatment of rabbit intestinal epithelial cells with lipopolysaccharide did not inhibit adherence of these strains. These results suggest that outer membrane proteins of V. parahaemolyticus play an important role in the adherence of Kanagawa-positive strains to rabbit intestinal epithelial cells.
用副溶血性弧菌神奈川现象阳性菌株的外膜制剂对家兔进行免疫，制备抗血清，并用其脂多糖进行吸收。抗外膜蛋白血清及其Fab（IgG）片段在体外可抑制神奈川阳性菌株对家兔肠上皮细胞的黏附。家兔肠上皮细胞与外膜制剂预孵育也可抑制这些细菌的黏附。抗脂多糖血清或其Fab（IgG）片段不能抑制副溶血性弧菌的黏附。此外，用脂多糖预处理家兔肠上皮细胞并不能抑制这些菌株的黏附。这些结果表明，副溶血性弧菌的外膜蛋白在神奈川阳性菌株对家兔肠上皮细胞的黏附中起重要作用。
