Carruthers M M
J Infect Dis. 1977 Oct;136(4):588-92. doi: 10.1093/infdis/136.4.588.
Kanagawa-positive Vibrio parahaemolyticus adhered rapidly to suspension-grown HeLa cells and to human fetal intestinal (HFI) cells in monolayer. Kanagawa-negative V. parahaemolyticus did not adhere to HeLa cells and adhered to HFI cells at a much slower rate than Kanagawa-positive bacteria. In the HFI cell system, adherence was dependent on the presence of calcium in the incubation mixture. It was unaffected by pretreatment of the HFI cell monolayer with neuraminidase or potassium periodate. Adherence was unaffected by neuraminidase or ultraviolet irradiation of bacteria but was decreased by heat, ethanol, or periodate pretreatment. These results suggest that adherence of Kanagawa-positive V. parahaemolyticus to epithelial cells in vitro depends on interaction between the cell surface of the bacteria and the epithelial cell and that a carbohydrate on the outer membrane of the bacterial cell wall is necessary for this interaction.
神奈川阳性副溶血性弧菌能迅速黏附于悬浮生长的HeLa细胞以及单层培养的人胎儿肠道（HFI）细胞。神奈川阴性副溶血性弧菌不黏附HeLa细胞，且与神奈川阳性菌相比，其黏附HFI细胞的速率要慢得多。在HFI细胞系统中，黏附依赖于孵育混合物中钙的存在。用神经氨酸酶或高碘酸钾预处理HFI细胞单层对黏附没有影响。细菌经神经氨酸酶处理或紫外线照射后黏附不受影响，但经加热、乙醇或高碘酸钾预处理后黏附减少。这些结果表明，体外神奈川阳性副溶血性弧菌对上皮细胞的黏附取决于细菌细胞表面与上皮细胞之间的相互作用，并且细菌细胞壁外膜上的一种碳水化合物对于这种相互作用是必需的。
