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2
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本文引用的文献

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Conjugative Transfer by the Virulence System of Agrobacterium tumefaciens.农杆菌毒力系统的共轭转移。
Science. 1992 May 29;256(5061):1324-7. doi: 10.1126/science.256.5061.1324.
2
The cytoplasmic DNA-binding protein TraM binds to the inner membrane protein TraD in vitro.细胞质DNA结合蛋白TraM在体外与内膜蛋白TraD结合。
J Bacteriol. 1997 Oct;179(19):6133-7. doi: 10.1128/jb.179.19.6133-6137.1997.
3
Genetic evidence of a coupling role for the TraG protein family in bacterial conjugation.TraG蛋白家族在细菌接合中起耦合作用的遗传证据。
Mol Gen Genet. 1997 Apr 28;254(4):400-6. doi: 10.1007/s004380050432.
4
The mating pair formation system of plasmid RP4 defined by RSF1010 mobilization and donor-specific phage propagation.由RSF1010转移和供体特异性噬菌体繁殖所定义的质粒RP4的配对形成系统。
J Bacteriol. 1993 Oct;175(20):6415-25. doi: 10.1128/jb.175.20.6415-6425.1993.
5
Genetic organization of the conjugal DNA processing region of the IncW plasmid R388.IncW质粒R388接合DNA加工区域的遗传组织
J Mol Biol. 1994 Jan 14;235(2):448-64. doi: 10.1006/jmbi.1994.1005.
6
Requirements for mobilization of plasmids RSF1010 and ColE1 by the IncW plasmid R388: trwB and RP4 traG are interchangeable.IncW质粒R388对质粒RSF1010和ColE1的动员要求：trwB和RP4 traG可互换。
J Bacteriol. 1994 Jul;176(14):4455-8. doi: 10.1128/jb.176.14.4455-4458.1994.
7
Essential motifs of relaxase (TraI) and TraG proteins involved in conjugative transfer of plasmid RP4.参与质粒RP4接合转移的松弛酶（TraI）和TraG蛋白的必需基序。
J Bacteriol. 1994 Jul;176(14):4285-95. doi: 10.1128/jb.176.14.4285-4295.1994.
8
Genetic and molecular characterization of Tn21, a multiple resistance transposon from R100.1.来自R100.1的多重耐药转座子Tn21的遗传和分子特征分析
J Bacteriol. 1982 Jul;151(1):222-28. doi: 10.1128/jb.151.1.222-228.1982.
9
A system to study promoter and terminator signals recognized by Escherichia coli RNA polymerase.一种用于研究大肠杆菌RNA聚合酶识别的启动子和终止子信号的系统。
Gene Amplif Anal. 1981;2:383-415.
10
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.改良的M13噬菌体克隆载体和宿主菌株：M13mp18和pUC19载体的核苷酸序列。
Gene. 1985;33(1):103-19. doi: 10.1016/0378-1119(85)90120-9.

蛋白质TraD的羧基末端增加了F质粒接合转移的特异性和效率。

The carboxyl terminus of protein TraD adds specificity and efficiency to F-plasmid conjugative transfer.

作者信息

Sastre J I, Cabezón E, de la Cruz F

机构信息

Department of Molecular Biology, University of Cantabria, 39011 Santander, Spain.

出版信息

J Bacteriol. 1998 Nov;180(22):6039-42. doi: 10.1128/JB.180.22.6039-6042.1998.

DOI:10.1128/JB.180.22.6039-6042.1998
PMID:9811665
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC107681/
Abstract

We isolated and characterized traD mutants with an altered specificity of interaction with relaxosomes of various conjugative (F and R388) and mobilizable (RSF1010 and ColE1) plasmids. The change in specificity was due to a loss of some amino acids in the carboxyl terminus of TraD that resulted in a broadening of the range of mobilizable relaxosomes at the expense of a decrease in the efficiency of F-plasmid transfer.

摘要

我们分离并鉴定了traD突变体，其与各种接合型（F和R388）和可移动型（RSF1010和ColE1）质粒的松弛体相互作用的特异性发生了改变。特异性的变化是由于TraD羧基末端一些氨基酸的缺失，这导致可移动松弛体范围变宽，但以F质粒转移效率降低为代价。