细胞质DNA结合蛋白TraM在体外与内膜蛋白TraD结合。
The cytoplasmic DNA-binding protein TraM binds to the inner membrane protein TraD in vitro.
作者信息
Disqué-Kochem C, Dreiseikelmann B
机构信息
Mikrobiologie/Gentechnologie, Universität Bielefeld, Germany.
出版信息
J Bacteriol. 1997 Oct;179(19):6133-7. doi: 10.1128/jb.179.19.6133-6137.1997.
Abstract
The cytoplasmic protein TraM is one of four essential gene products of the F factor which are involved in DNA transfer after mating pair formation. TraM binds to three specific sites within the oriT region. Besides regulation of its own synthesis, the precise function of TraM during conjugation is not yet known. In the present work, the affinity of TraM to TraD was studied in vitro by an overlay assay and by affinity chromatography. Whether the interaction between TraM and TraD causes a transient or permanent anchoring of the F factor to the site of transfer is discussed. A 35-kDa host membrane protein of yet unknown function also shows affinity to TraM and may be involved in this anchoring process as well.
摘要
细胞质蛋白TraM是F因子的四个必需基因产物之一，参与配对形成后DNA的转移。TraM与oriT区域内的三个特定位点结合。除了对自身合成的调控外，TraM在接合过程中的精确功能尚不清楚。在本研究中，通过覆盖分析和亲和色谱法在体外研究了TraM与TraD的亲和力。讨论了TraM与TraD之间的相互作用是否导致F因子在转移位点的短暂或永久锚定。一种功能未知的35 kDa宿主膜蛋白也显示出与TraM的亲和力，可能也参与了这一锚定过程。
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