• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Localization of a baculovirus-induced chitinase in the insect cell endoplasmic reticulum.杆状病毒诱导的几丁质酶在昆虫细胞内质网中的定位
J Virol. 1998 Dec;72(12):10207-12. doi: 10.1128/JVI.72.12.10207-10212.1998.
2
Partial redistribution of the Autographa californica nucleopolyhedrovirus chitinase in virus-infected cells accompanies mutation of the carboxy-terminal KDEL ER-retention motif.苜蓿银纹夜蛾核型多角体病毒几丁质酶在病毒感染细胞中的部分重新分布伴随着羧基末端KDEL内质网保留基序的突变。
J Gen Virol. 2002 Mar;83(Pt 3):685-694. doi: 10.1099/0022-1317-83-3-685.
3
Deletion of the Autographa californica nucleopolyhedrovirus chitinase KDEL motif and in vitro and in vivo analysis of the modified virus.
J Gen Virol. 2004 Apr;85(Pt 4):821-831. doi: 10.1099/vir.0.19732-0.
4
Mutagenesis of the active site coding region of the Autographa californica nucleopolyhedrovirus chiA gene.苜蓿银纹夜蛾核型多角体病毒chiA基因活性位点编码区域的诱变
J Gen Virol. 2000 May;81(Pt 5):1403-11. doi: 10.1099/0022-1317-81-5-1403.
5
Liquefaction of Autographa californica nucleopolyhedrovirus-infected insects is dependent on the integrity of virus-encoded chitinase and cathepsin genes.苜蓿银纹夜蛾核型多角体病毒感染的昆虫的液化取决于病毒编码的几丁质酶和组织蛋白酶基因的完整性。
Virology. 1997 Nov 24;238(2):243-53. doi: 10.1006/viro.1997.8816.
6
Interaction of Autographa californica multiple nucleopolyhedrovirus cathepsin protease progenitor (proV-CATH) with insect baculovirus chitinase as a mechanism for proV-CATH cellular retention.美洲棉铃虫多核多角体病毒组织蛋白酶前体(proV-CATH)与昆虫杆状病毒几丁质酶的相互作用是 proV-CATH 细胞保留的机制。
J Virol. 2011 Apr;85(8):3918-29. doi: 10.1128/JVI.02165-10. Epub 2011 Feb 2.
7
Lack of involvement of haemocytes in the establishment and spread of infection in Spodoptera frugiperda larvae infected with the baculovirus Autographa californica M nucleopolyhedrovirus by intrahaemocoelic injection.通过血腔注射感染苜蓿银纹夜蛾多核衣壳核型多角体病毒的草地贪夜蛾幼虫，血细胞未参与杆状病毒感染的建立和传播。
J Gen Virol. 2002 Jul;83(Pt 7):1565-1572. doi: 10.1099/0022-1317-83-7-1565.
8
Chitinase from Autographa californica multiple nucleopolyhedrovirus: rapid purification from Sf-9 medium and mode of action.来自苜蓿银纹夜蛾多粒包埋核型多角体病毒的几丁质酶：从 Sf-9 培养基中快速纯化及作用模式
Biosci Biotechnol Biochem. 2011;75(9):1763-9. doi: 10.1271/bbb.110300. Epub 2011 Sep 7.
9
Identification and preliminary characterization of a chitinase gene in the Autographa californica nuclear polyhedrosis virus genome.苜蓿银纹夜蛾核型多角体病毒基因组中一个几丁质酶基因的鉴定与初步表征
Virology. 1995 Oct 1;212(2):673-85. doi: 10.1006/viro.1995.1525.
10
Autographa californica M nucleopolyhedrovirus early GP64 synthesis mitigates developmental resistance in orally infected noctuid hosts.苜蓿银纹夜蛾多核衣壳核型多角体病毒早期GP64合成减轻了经口感染的夜蛾科宿主的发育抗性。
J Gen Virol. 2004 Apr;85(Pt 4):833-842. doi: 10.1099/vir.0.19773-0.

引用本文的文献

1
The Bioengineering of Insect Cell Lines for Biotherapeutics and Vaccine Production: An Updated Review.用于生物治疗和疫苗生产的昆虫细胞系生物工程：最新综述
Vaccines (Basel). 2025 May 23;13(6):556. doi: 10.3390/vaccines13060556.
2
StaufenC facilitates utilization of the ERAD pathway to transport dsRNA through the endoplasmic reticulum to the cytosol.StaufenC 促进利用 ERAD 途径将 dsRNA 通过内质网运输到细胞质中。
Proc Natl Acad Sci U S A. 2024 Jun 25;121(26):e2322927121. doi: 10.1073/pnas.2322927121. Epub 2024 Jun 17.
3
A distinct lineage of giant viruses brings a rhodopsin photosystem to unicellular marine predators.一种独特的巨型病毒谱系为单细胞海洋捕食者带来了视紫红质光系统。
Proc Natl Acad Sci U S A. 2019 Oct 8;116(41):20574-20583. doi: 10.1073/pnas.1907517116. Epub 2019 Sep 23.
4
Improving the baculovirus expression vector system with vankyrin-enhanced technology.利用泛素结构域蛋白增强技术改进杆状病毒表达载体系统。
Biotechnol Prog. 2017 Nov;33(6):1496-1507. doi: 10.1002/btpr.2516. Epub 2017 Jul 6.
5
Phosphorylation Induces Structural Changes in the Autographa californica Nucleopolyhedrovirus P10 Protein.磷酸化诱导苜蓿银纹夜蛾核型多角体病毒P10蛋白的结构变化。
J Virol. 2017 Jun 9;91(13). doi: 10.1128/JVI.00002-17. Print 2017 Jul 1.
6
Improved Production Efficiency of Virus-Like Particles by the Baculovirus Expression Vector System.杆状病毒表达载体系统提高病毒样颗粒的生产效率
PLoS One. 2015 Oct 12;10(10):e0140039. doi: 10.1371/journal.pone.0140039. eCollection 2015.
7
Ultra Deep Sequencing of a Baculovirus Population Reveals Widespread Genomic Variations.杆状病毒群体的超深度测序揭示了广泛的基因组变异。
Viruses. 2015 Jul 7;7(7):3625-46. doi: 10.3390/v7072788.
8
Expression of the Cydia pomonella granulovirus matrix metalloprotease enhances Autographa californica multiple nucleopolyhedrovirus virulence and can partially substitute for viral cathepsin.苹果蠹蛾颗粒体病毒基质金属蛋白酶的表达增强了苜蓿银纹夜蛾多核多角体病毒的毒力，并可部分替代病毒组织蛋白酶。
Virology. 2015 Jul;481:166-78. doi: 10.1016/j.virol.2015.02.022. Epub 2015 Mar 17.
9
Reaching the melting point: Degradative enzymes and protease inhibitors involved in baculovirus infection and dissemination.达到熔点：参与杆状病毒感染和传播的降解酶与蛋白酶抑制剂
Virology. 2015 May;479-480:637-49. doi: 10.1016/j.virol.2015.01.027. Epub 2015 Feb 25.
10
Identification of functionally important residues of the silkmoth pheromone biosynthesis-activating neuropeptide receptor, an insect ortholog of the vertebrate neuromedin U receptor.家蚕性信息素生物合成激活神经肽受体功能重要残基的鉴定，该受体是脊椎动物神经介素U受体的昆虫直系同源物。
J Biol Chem. 2014 Jul 4;289(27):19150-63. doi: 10.1074/jbc.M113.488999. Epub 2014 May 20.

本文引用的文献

1
Regulation of processing of a plant glycoprotein in the Golgi complex: A comparative study usingXenopus oocytes.植物糖蛋白在高尔基复合体中的加工调控：使用非洲爪蟾卵母细胞的比较研究。
Planta. 1986 Mar;169(1):108-16. doi: 10.1007/BF01369781.
2
Liquefaction of Autographa californica nucleopolyhedrovirus-infected insects is dependent on the integrity of virus-encoded chitinase and cathepsin genes.苜蓿银纹夜蛾核型多角体病毒感染的昆虫的液化取决于病毒编码的几丁质酶和组织蛋白酶基因的完整性。
Virology. 1997 Nov 24;238(2):243-53. doi: 10.1006/viro.1997.8816.
3
Genetically variable triplet repeats in a RING-finger ORF of Helicoverpa species baculoviruses.
Virus Res. 1997 May;49(1):67-77. doi: 10.1016/s0168-1702(97)01454-8.
4
The sequence of the Orgyia pseudotsugata multinucleocapsid nuclear polyhedrosis virus genome.云杉芽卷叶蛾多核衣壳核型多角体病毒基因组序列。
Virology. 1997 Mar 17;229(2):381-99. doi: 10.1006/viro.1997.8448.
5
The complete DNA sequence of Autographa californica nuclear polyhedrosis virus.苜蓿银纹夜蛾核型多角体病毒的完整DNA序列。
Virology. 1994 Aug 1;202(2):586-605. doi: 10.1006/viro.1994.1380.
6
Authentic processing and targeting of active maize auxin-binding protein in the baculovirus expression system.杆状病毒表达系统中活性玉米生长素结合蛋白的真实加工与靶向
Plant Physiol. 1994 Aug;105(4):1049-57. doi: 10.1104/pp.105.4.1049.
7
Characterization of v-cath, a cathepsin L-like proteinase expressed by the baculovirus Autographa californica multiple nuclear polyhedrosis virus.杆状病毒苜蓿银纹夜蛾多核型多角体病毒所表达的组织蛋白酶L样蛋白酶v-cath的特性分析
J Gen Virol. 1995 May;76 ( Pt 5):1091-8. doi: 10.1099/0022-1317-76-5-1091.
8
Crystal structure of a bacterial chitinase at 2.3 A resolution.分辨率为2.3埃的细菌几丁质酶晶体结构。
Structure. 1994 Dec 15;2(12):1169-80. doi: 10.1016/s0969-2126(94)00119-7.
9
Identification and preliminary characterization of a chitinase gene in the Autographa californica nuclear polyhedrosis virus genome.苜蓿银纹夜蛾核型多角体病毒基因组中一个几丁质酶基因的鉴定与初步表征
Virology. 1995 Oct 1;212(2):673-85. doi: 10.1006/viro.1995.1525.
10
A C-terminal signal prevents secretion of luminal ER proteins.C 末端信号可阻止内质网腔蛋白的分泌。
Cell. 1987 Mar 13;48(5):899-907. doi: 10.1016/0092-8674(87)90086-9.

杆状病毒诱导的几丁质酶在昆虫细胞内质网中的定位

Localization of a baculovirus-induced chitinase in the insect cell endoplasmic reticulum.

作者信息

Thomas C J, Brown H L, Hawes C R, Lee B Y, Min M K, King L A, Possee R D

机构信息

NERC Institute of Virology and Environmental Microbiology, Oxford OX1 3SR, United Kingdom.

出版信息

J Virol. 1998 Dec;72(12):10207-12. doi: 10.1128/JVI.72.12.10207-10212.1998.

DOI:10.1128/JVI.72.12.10207-10212.1998
PMID:9811762
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC110568/
Abstract

Confocal immunofluorescence microscopy was used to demonstrate that the Autographa californica nucleopolyhedrovirus (AcMNPV) chitinase was localized within the endoplasmic reticulum (ER) of virus-infected insect cells. This was consistent with removal of the signal peptide from the chitinase and an ER localization motif (KDEL) at the carboxyl end of the protein. Chitinase release from cells, a prerequisite for liquefaction of virus-infected insect larvae, appears to be aided by synthesis of the p10 protein. Deletion of p10 from the AcMNPV genome delayed the appearance of chitinase activity in the medium of virus-infected cells by 24 h and also delayed liquefaction of virus-infected Trichoplusia ni larvae by the same period.

摘要

共聚焦免疫荧光显微镜用于证明苜蓿银纹夜蛾核多角体病毒（AcMNPV）几丁质酶定位于病毒感染昆虫细胞的内质网（ER）中。这与几丁质酶信号肽的去除以及该蛋白质羧基末端的内质网定位基序（KDEL）一致。几丁质酶从细胞中释放是病毒感染昆虫幼虫液化的前提条件，这似乎得益于p10蛋白的合成。从AcMNPV基因组中删除p10会使病毒感染细胞培养基中几丁质酶活性的出现延迟24小时，同时也会使病毒感染的粉纹夜蛾幼虫的液化延迟相同时间。