Gall J G, Crystal R G, Falck-Pedersen E
Department of Microbiology, W. R. Hearst Research Foundation, Cornell University Medical College, New York, New York 10021, USA.
J Virol. 1998 Dec;72(12):10260-4. doi: 10.1128/JVI.72.12.10260-10264.1998.
This study has used the strategy of gene replacement to characterize the contribution of the adenovirus (Ad) capsid protein hexon to serotype definition. By replacing the Ad type 5 (Ad5) hexon gene with sequences from Ad2, we have changed the type specificity of the chimeric virus. The type-determining epitopes are primarily associated with loop 1 of hexon and, to a much lesser degree, with loop 2. In spite of the serotype distinctiveness of the chimeric hexon viruses, epitope similarity between the vectors resulted in a low level of cross-reactive neutralizing antibody, which in combination with activated cellular and innate arms of the immune system is sufficient to suppress gene transduction following readministration in vivo.
本研究采用基因置换策略来表征腺病毒（Ad）衣壳蛋白六邻体对血清型定义的贡献。通过用Ad2的序列替换Ad5六邻体基因，我们改变了嵌合病毒的型特异性。决定血清型的表位主要与六邻体的环1相关，在较小程度上与环2相关。尽管嵌合六邻体病毒具有血清型独特性，但载体之间的表位相似性导致交叉反应性中和抗体水平较低，这与免疫系统的激活细胞和固有分支相结合，足以在体内再次给药后抑制基因转导。
