Broda D M, Boerema J A, Bell R G
MIRINZ Food Technology and Research, Hamilton, New Zealand.
Lett Appl Microbiol. 1998 Oct;27(4):219-23. doi: 10.1046/j.1472-765x.1998.00420.x.
Isolates (259) of psychrotrophic Clostridium spp. associated with either blown pack spoilage (five isolates) or slaughter stock (254 isolates) were screened for the presence of botulinum neurotoxin (BoNT) genes using degenerate PCR primers capable of amplifying A, B, E, F and G BoNT genes. No BoNT gene amplification products were detected using DNA templates from the 259 psychrotrophic isolates, including 249 isolates that showed the same 16S rRNA gene Restriction Fragment Length Polymorphism (RFLP) patterns as authentic Cl. botulinum type B. It is concluded that although the growth of such micro-organisms in vacuum-packed chilled meat leads to product spoilage, it does not prejudice product safety.
使用能够扩增A、B、E、F和G型肉毒杆菌神经毒素（BoNT）基因的简并PCR引物，对与胀袋腐败（5株分离株）或屠宰牲畜（254株分离株）相关的嗜冷梭菌属分离株（共259株）进行肉毒杆菌神经毒素（BoNT）基因筛选。使用来自这259株嗜冷分离株的DNA模板未检测到BoNT基因扩增产物，其中包括249株与正宗B型肉毒梭菌显示相同16S rRNA基因限制性片段长度多态性（RFLP）模式的分离株。得出的结论是，尽管此类微生物在真空包装的冷藏肉中生长会导致产品腐败，但不会损害产品安全性。
