Liu J, Wang P Y, Luo D C, Yu Z H, Sun B Y
Department of Pathophysiology, Third Military Medical College, Chongqing.
Sheng Li Xue Bao. 1997 Jun;49(3):292-8.
The effects of hypoxia on the bovine pulmonary arterial smooth muscle cell (PASM) proliferation and serotonin transporter gene expression were investigated using 3H-Thymidine incorporation, immunocytochemical staining and mRNA dot blot hybridyzation, the results were as follows, PASM 3H-Thymidine incorporation increased in response to anoxia for 24 h (P < 0.05). The anoxia-induced increased in DNA synthesis of PASM was significantly stimulated by 5-HT (100, 10 and 1 mumol/L) (P < 0.001), but 5-HT did not increase 3H-TdR incorporation under normoxic condition. Immunocytochemical reaction of serotonin in PASM under normoxic condition was markedly stronger than that of anoxic PASM (P < 0.001). Dot blot analysis showed hypoxia increased serotonin transporter mRNA expression of PASM (P < 0.01). These results indicate that stimulation of PASM proliferation with enhanced serotonin transporter gene expression and high consumption of 5-HT in hypoxia or/and anoxia may take part in the development of hypoxic pulmonany hypertention.
采用3H-胸腺嘧啶核苷掺入法、免疫细胞化学染色法及mRNA斑点杂交法，研究缺氧对牛肺动脉平滑肌细胞（PASM）增殖及5-羟色胺转运体基因表达的影响，结果如下：缺氧24小时后，PASM的3H-胸腺嘧啶核苷掺入量增加（P<0.05）。5-羟色胺（5-HT，100、10和1μmol/L）可显著刺激缺氧诱导的PASM DNA合成增加（P<0.001），但在常氧条件下5-HT不会增加3H-胸腺嘧啶核苷掺入量。常氧条件下PASM中5-羟色胺的免疫细胞化学反应明显强于缺氧PASM（P<0.001）。斑点杂交分析显示，缺氧可增加PASM的5-羟色胺转运体mRNA表达（P<0.01）。这些结果表明，在缺氧或/和无氧状态下，PASM增殖受刺激、5-羟色胺转运体基因表达增强以及5-HT高消耗可能参与了缺氧性肺动脉高压的发生发展。
