Wang P, Liu J, Liao W, Xu S, Luo D, Sun B
Department of Pathophysiology, Third Military Medical College, Chongqing.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 1997 Aug;13(3):209-12.
Vascular endothelial cell are closely related to vascular smooth muscle cells in structure and function. The interactions between them may play important roles in the modulation of function and structure of vascular wall. In the present study, the mitogenic regulations between cultured new bovine pulmonary arterial endothelial cell (PAEC) and pulmonary arterial smooth muscle (PASM) were investigated. When PAECs and PASMs were mixcultured, 3H-TdR incorporation into the mixed cells decreased significantly (P < 0.001 vs control). When PAECs and PASMs were cultured in conditioned medium from PASMs and PAECs respectively or they were cocultured, the proliferation of PAECs was inhibited while that of PASM was stimulated significantly (P < 0.05 vs control). It was also found that the concentration of cAMP increased but cGMP decreased in cocultured PASMs (P < 0.01 vs control), while the concentration of both cAMP and cGMP decreased significantly in cocultured PAECs (P < 0.01 vs control). These findings suggest that PAECs and PASMs may regulate their proliferation each other through the second messenger system.
血管内皮细胞与血管平滑肌细胞在结构和功能上密切相关。它们之间的相互作用可能在血管壁功能和结构的调节中发挥重要作用。在本研究中，对培养的新生牛肺动脉内皮细胞（PAEC）和肺动脉平滑肌（PASM）之间的促有丝分裂调节进行了研究。当PAEC和PASM混合培养时，混合细胞中3H-TdR掺入量显著降低（与对照组相比，P < 0.001）。当PAEC和PASM分别在来自PASM和PAEC的条件培养基中培养或共培养时，PAEC的增殖受到抑制，而PASM的增殖则受到显著刺激（与对照组相比，P < 0.05）。还发现共培养的PASM中cAMP浓度升高而cGMP浓度降低（与对照组相比，P < 0.01），而共培养的PAEC中cAMP和cGMP浓度均显著降低（与对照组相比，P < 0.01）。这些发现表明，PAEC和PASM可能通过第二信使系统相互调节它们的增殖。
