酿酒酵母RNA聚合酶II全酶中新调节亚基的鉴定。
Identification of new mediator subunits in the RNA polymerase II holoenzyme from Saccharomyces cerevisiae.
作者信息
Gustafsson C M, Myers L C, Beve J, Spåhr H, Lui M, Erdjument-Bromage H, Tempst P, Kornberg R D
机构信息
Department of Clinical Chemistry and Transfusion Medicine, Göteborg University, Bruna Stråket 16, 413 45 Göteborg, Sweden.
出版信息
J Biol Chem. 1998 Nov 20;273(47):30851-4. doi: 10.1074/jbc.273.47.30851.
Mediator was isolated from yeast on the basis of its requirement for transcriptional activation in a fully defined system. We have now identified three new members of mediator in the low molecular mass range by peptide sequence determination. These are the products of the NUT2, CSE2, and MED11 genes. The product of the NUT1 gene is evidently a component of mediator as well. NUT1 and NUT2 were earlier identified as negative regulators of the HO promoter, whereas mutations in CSE2 affect chromosome segregation. MED11 is a previously uncharacterized gene. The existence of these proteins in the mediator complex was verified by copurification and co-immunoprecipitation with RNA polymerase II holoenzyme.
中介体是在一个完全确定的系统中，基于其对转录激活的需求从酵母中分离出来的。我们现在通过肽序列测定，在低分子量范围内鉴定出了中介体的三个新成员。它们是NUT2、CSE2和MED11基因的产物。NUT1基因的产物显然也是中介体的一个组成部分。NUT1和NUT2早期被鉴定为HO启动子的负调控因子，而CSE2中的突变会影响染色体分离。MED11是一个先前未被表征的基因。通过与RNA聚合酶II全酶的共纯化和共免疫沉淀，证实了这些蛋白质存在于中介体复合物中。
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