[蛋白质E 98 - 113序列是蜱传脑炎病毒与细胞膜的融合位点]

[Protein E 98-113 sequence is a fusion site of tick-borne encephalitis virus with cellular membrane].

作者信息

Volkova T D, Vol'pina O M, Ivanov V T, Vargin V V, Vorovich M F, Timofeev A V, Semenov B F, Tsekhanovskaia N A, Pressman E K

机构信息

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.

出版信息

Bioorg Khim. 1998 Sep;24(9):676-81.

PMID:9813732

Abstract

The synthetic peptide with the conservative 98-113 sequence of protein E of tick-borne encephalitis virus was studied in order to elucidate its role in the functioning of flaviviruses. The peptide was shown to inhibit the in vitro infection of macrophages with the virus. An antibody that specifically binds this peptide was found among the set of monoclonal antibodies produced against protein E. This antibody was found to prevent penetration of the virus into liposomes. A correlation was found between our results and data on the spatial structure of protein E and its interspecies homology. The protein E 98-113 sequence of the tick-borne encephalitis virus was found to be the fusion site of the viral envelope with a cellular membrane.

摘要

为了阐明其在黄病毒功能中的作用，对具有蜱传脑炎病毒E蛋白保守98 - 113序列的合成肽进行了研究。结果表明，该肽可抑制病毒对巨噬细胞的体外感染。在针对E蛋白产生的一组单克隆抗体中，发现了一种能特异性结合该肽的抗体。发现该抗体可阻止病毒进入脂质体。我们的结果与E蛋白的空间结构及其种间同源性数据之间存在相关性。发现蜱传脑炎病毒的E蛋白98 - 113序列是病毒包膜与细胞膜的融合位点。