Al-Tawfiq J A, Thornton A C, Katz B P, Fortney K R, Todd K D, Hood A F, Spinola S M
Departments of Medicine, Microbiology, and Immunology, Indiana University, Indianapolis, IN 46202, USA.
J Infect Dis. 1998 Dec;178(6):1684-7. doi: 10.1086/314483.
Human volunteers were challenged with Haemophilus ducreyi. Twenty subjects were inoculated with 2 doses (approximately 30 cfu) of live and 1 dose of heat-killed bacteria at 3 sites on the arm. Eight subjects were assigned to biopsy 1 or 4 days after inoculation, and 12 were biopsied after they developed a painful pustular lesion or were followed until disease resolved. Papules developed at 95% of 40 sites infected with live bacteria (95% confidence interval [CI], 83. 1%-99.4%). In 24 sites followed to end point, 27% of the papules resolved, 69% (95% CI, 47.1%-86.6%) evolved into pustules, and 4% remained at the papular stage. Recovery rates of H. ducreyi from surface cultures ranged from 13% to 41%. H. ducreyi was recovered from biopsies of 12 of 15 pustules and 1 of 7 papules, suggesting that H. ducreyi replicates between the papular and pustular stages of disease.
人类志愿者受到杜克雷嗜血杆菌的攻击。20名受试者在手臂的3个部位接种了2剂（约30 cfu）活菌和1剂热灭活菌。8名受试者在接种后1天或4天接受活检，12名受试者在出现疼痛性脓疱病变后接受活检，或随访至疾病消退。在40个感染活菌的部位中，95%出现丘疹（95%置信区间[CI]，83.1%-99.4%）。在24个随访至终点的部位，27%的丘疹消退，69%（95%CI，47.1%-86.6%）演变为脓疱，4%停留在丘疹阶段。从表面培养物中回收杜克雷嗜血杆菌的比率在13%至41%之间。从15个脓疱中的12个和7个丘疹中的1个活检样本中回收了杜克雷嗜血杆菌，这表明杜克雷嗜血杆菌在疾病的丘疹和脓疱阶段之间进行复制。
