Wakefield L M, Letterio J J, Chen T, Danielpour D, Allison R S, Pai L H, Denicoff A M, Noone M H, Cowan K H, O'Shaughnessy J A
Laboratory of Chemoprevention and Medicine Branch, National Cancer Institute, Bethesda, Maryland 20892-5055, USA.
Clin Cancer Res. 1995 Jan;1(1):129-36.
A method has been developed to determine true plasma transforming growth factor beta (TGF-beta) levels by using the platelet alpha granule-specific marker, platelet factor 4, to correct for the TGF-beta contributed by platelets degranulated ex vivo. TGF-beta levels were measured on acid-ethanol extracts of human plasma using isoform-specific sandwich enzyme-linked immunosorbent assays. Normal human subjects had 4.1 +/- 2.0 ng/ml TGF-beta1 (range, 2.0-12.0; n = 42), <0.2 ng/ml TGF-beta2, and <0.1 ng/ml TGF-beta3 in their plasma. There were no significant changes with age or with hormonal status, but any given individual showed fluctuations of up to 3-fold in measured plasma TGF-beta levels due to unknown factors. Of 28 patients with advanced metastatic breast cancer, 2 had greatly elevated TGF-beta1 levels, while the rest were in the normal range. The presence of physiologically significant levels of TGF-beta1 in the plasmas of normal human subjects may indicate previously unsuspected endocrine roles for this peptide, while TGF-beta2 and TGF-beta3 appear to act only in a local autocrine/paracrine fashion.
已开发出一种方法，通过使用血小板α颗粒特异性标志物血小板因子4来校正离体脱颗粒血小板所贡献的转化生长因子β（TGF-β），从而确定真实的血浆TGF-β水平。使用亚型特异性夹心酶联免疫吸附测定法测量人血浆酸乙醇提取物中的TGF-β水平。正常人类受试者血浆中TGF-β1为4.1±2.0 ng/ml（范围2.0 - 12.0；n = 42），TGF-β2<0.2 ng/ml，TGF-β3<0.1 ng/ml。年龄或激素状态无显著变化，但由于未知因素，任何个体测得的血浆TGF-β水平波动可达3倍。在28例晚期转移性乳腺癌患者中，2例TGF-β1水平大幅升高，其余患者在正常范围内。正常人类受试者血浆中存在生理显著水平的TGF-β1可能表明该肽以前未被怀疑的内分泌作用，而TGF-β2和TGF-β3似乎仅以局部自分泌/旁分泌方式起作用。
