Rotsch C, Braet F, Wisse E, Radmacher M
Lehrstuhl für Angewandte Physik, Universität München, Germany.
Cell Biol Int. 1997 Nov;21(11):685-96. doi: 10.1006/cbir.1997.0213.
The authors investigated the morphology and the elastic properties of living cultured rat liver macrophages (Kupffer cells) with an atomic force microscope (AFM). Continuous imaging and elasticity mapping of individual cells in physiological buffer was carried out for several hours without damaging the cells as judged by their persistent undisturbed morphology. Dynamic events such as protrusive activity were observed in time course. The importance of the cytoskeleton for the mechanical properties of the cell has been investigated by measuring the cell's elasticity as a function of position. Chemical disassembly of the actin network by applying 10 microgram/ml cytochalasin B decreased the cell's average elastic modulus seven-fold within less than 40 minutes. Treating the cells with 0.1 micrograms/ml latrunculin A resulted in a two-fold decrease in the elastic modulus merely in the perinuclear region after 40 minutes, whereas other parts of the cell were not affected.
作者使用原子力显微镜（AFM）研究了培养的活大鼠肝巨噬细胞（库普弗细胞）的形态和弹性特性。在生理缓冲液中对单个细胞进行连续成像和弹性图谱分析，持续数小时，根据细胞持续未受干扰的形态判断，细胞未受到损伤。在时间进程中观察到诸如突出活动等动态事件。通过测量细胞弹性随位置的变化，研究了细胞骨架对细胞力学性能的重要性。应用10微克/毫升细胞松弛素B对肌动蛋白网络进行化学拆解，在不到40分钟内使细胞的平均弹性模量降低了七倍。用0.1微克/毫升拉春库林A处理细胞40分钟后，仅在核周区域弹性模量降低了两倍，而细胞的其他部分未受影响。
