沙眼衣原体临床分离株在组织培养中连续传代对omp1序列的影响。
Effect of serial passage in tissue culture on sequence of omp1 from Chlamydia trachomatis clinical isolates.
作者信息
Stothard D R, Van Der Pol B, Smith N J, Jones R B
机构信息
Department of Medicine, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA.
出版信息
J Clin Microbiol. 1998 Dec;36(12):3686-8. doi: 10.1128/JCM.36.12.3686-3688.1998.
Abstract
Previous epidemiological studies of Chlamydia trachomatis frequently have required expansion of isolates in tissue culture. The possibility that C. trachomatis omp1 might undergo mutation during such expansion has not been examined systematically. We found no differences in the omp1 sequences from 10 clinical specimens before and after 20 in vitro passages.
摘要
先前对沙眼衣原体的流行病学研究常常需要在组织培养中扩增分离株。沙眼衣原体omp1在这种扩增过程中可能发生突变的可能性尚未得到系统研究。我们发现，10份临床标本的omp1序列在20次体外传代前后没有差异。
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