Kedziora-Kornatowska K, Luciak M
Department of Internal Medicine, Military Medical University, Lódź, Poland.
Biochem Mol Biol Int. 1998 Oct;46(3):577-83. doi: 10.1080/15216549800204102.
The aim of this study was to examine lipid peroxidation and activities of key antioxidant enzymes in kidneys of rats with streptozotocin (STZ)-induced diabetes and the effect of aminoguanidine on diabetes-induced alterations. Three groups, 6 rats each, were studied: control animals, not treated diabetic rats and rats treated with aminoguanidine (AG; 1 g/liter of drinking water). After 6 and 12 weeks the animals were sacrificed and lipid peroxidation products and activities of antioxidant enzymes were determined in their kidney homogenates. Malondialdehyde (MDA) content was significantly elevated and activities of SOD and catalase decreased in the kidneys of STZ-diabetic rats. AG treatment attenuated the increase in MDA content and diminutions of activities of SOD and catalase in the kidneys of diabetic rats. These results confirm oxidative stress in the kidney of rats with STZ diabetes and point to an antioxidant effect of AG in experimental diabetes.
本研究旨在检测链脲佐菌素（STZ）诱导的糖尿病大鼠肾脏中的脂质过氧化作用及关键抗氧化酶的活性，以及氨基胍对糖尿病诱导的改变的影响。研究了三组动物，每组6只大鼠：对照动物、未经治疗的糖尿病大鼠和用氨基胍（AG；1克/升饮用水）治疗的大鼠。6周和12周后处死动物，测定其肾脏匀浆中的脂质过氧化产物和抗氧化酶活性。STZ糖尿病大鼠肾脏中的丙二醛（MDA）含量显著升高，超氧化物歧化酶（SOD）和过氧化氢酶的活性降低。AG治疗减轻了糖尿病大鼠肾脏中MDA含量的增加以及SOD和过氧化氢酶活性的降低。这些结果证实了STZ糖尿病大鼠肾脏中的氧化应激，并表明AG在实验性糖尿病中具有抗氧化作用。
