Hubbell W L, Gross A, Langen R, Lietzow M A
Jules Stein Eye Institute, University of California, Los Angeles 90095, USA.
Curr Opin Struct Biol. 1998 Oct;8(5):649-56. doi: 10.1016/s0959-440x(98)80158-9.
Site-directed spin labeling of proteins is experiencing a phase of rapid technical evolution, application and evaluation. New strategies have been introduced for determining membrane protein topography, electrostatic potentials, the orientation of proteins at membrane surfaces and inter-residue distances. New applications include studies of beta strands, structure mapping using spin-spin interactions, domain motions in soluble proteins and extensive structural analysis of a number of membrane and soluble proteins.
蛋白质的定点自旋标记正经历着一个技术快速发展、应用和评估的阶段。已引入了新的策略来确定膜蛋白的拓扑结构、静电势、蛋白质在膜表面的取向以及残基间距离。新的应用包括对β链的研究、利用自旋-自旋相互作用进行结构映射、可溶性蛋白质中的结构域运动以及对多种膜蛋白和可溶性蛋白质的广泛结构分析。
