Kulkosky P J, Allison C T, Allison T G, Marquez L M, Mattson B J
Department of Psychology, University of Southern Colorado, Pueblo 81001-4901, USA.
Alcohol. 1998 Nov;16(4):305-9. doi: 10.1016/s0741-8329(98)00019-6.
Administration of the neuropeptide cholecystokinin (CCK) is known to reduce food and alcohol intake and preference. The food satiation effect of CCK is reportedly dependent on serotonergic neurotransmission. Administration of 8-OH-DPAT, a serotonin1A autoreceptor agonist, reduces the ability of CCK to inhibit feeding. We determined if CCK's alcohol satiation effect also depends on activity of serotonergic neurons by administering 8-OH-DPAT (120-240 microg/kg) to 23-h water-deprived female and male rats, followed 1 h later by i.p. injection of CCK (4 microg/kg) and 30-min access to 5% w/v ethanol. 8-OH-DPAT significantly (p < 0.05) interacted with CCK, and reduced CCK's ethanol satiation effect when given i.p. but increased CCK's effect when given s.c. Female rats showed this interaction of 8-OH-DPAT with CCK at a higher dose than males when given i.p., but females were more sensitive to s.c. 8-OH-DPAT's ability to reduce ethanol intake. Results are consistent with previous findings of dose-, sex-, and route-dependent biphasic effects of 8-OH-DPAT on feeding and ethanol intake. A partial dependence of CCK's alcohol satiation effect on serotonergic neurotransmission is revealed in this design.
已知给予神经肽胆囊收缩素（CCK）可减少食物和酒精的摄入量及偏好。据报道，CCK的食物饱腹感效应依赖于血清素能神经传递。给予血清素1A自身受体激动剂8-OH-DPAT可降低CCK抑制进食的能力。我们通过给23小时禁水的雌性和雄性大鼠注射8-OH-DPAT（120 - 240微克/千克），1小时后腹腔注射CCK（4微克/千克）并给予30分钟5% （w/v）乙醇的摄入机会，来确定CCK的酒精饱腹感效应是否也依赖于血清素能神经元的活动。8-OH-DPAT与CCK存在显著（p < 0.05）的相互作用，腹腔注射时会降低CCK的乙醇饱腹感效应，而皮下注射时则会增强CCK的效应。腹腔注射时，雌性大鼠在比雄性更高的剂量下出现8-OH-DPAT与CCK的这种相互作用，但雌性对皮下注射的8-OH-DPAT降低乙醇摄入量的能力更敏感。结果与先前关于8-OH-DPAT对进食和乙醇摄入的剂量、性别及途径依赖性双相效应的研究结果一致。本研究设计揭示了CCK的酒精饱腹感效应部分依赖于血清素能神经传递。
