胚胎去腺苷酸化元件依赖性去腺苷酸化通过含有AUU重复序列的顺式元件得到增强。
Embryo deadenylation element-dependent deadenylation is enhanced by a cis element containing AUU repeats.
作者信息
Audic Y, Omilli F, Osborne H B
机构信息
CNRS UPR 41, Université de Rennes I, Campus de Beaulieu, 35042 Rennes Cedex, France.
出版信息
Mol Cell Biol. 1998 Dec;18(12):6879-84. doi: 10.1128/MCB.18.12.6879.
Abstract
The deadenylation of maternal mRNAs in the Xenopus embryo is a sequence-specific process. One cis element that targets maternal mRNAs for deadenylation after fertilization is the embryo deadenylation element (EDEN). This element, composed of U/R repeats, is specifically bound by a protein, EDEN-BP. In the present study we show that the rate at which an RNA containing an EDEN is deadenylated can be increased by the presence of an additional cis element composed of three AUU repeats. This effect was observed for a natural EDEN (c-mos) and two synthetic EDENs. Hence, the enhancement of EDEN-dependent deadenylation conferred by the (AUU)3 motif is not due to an interaction with a particular EDEN sequence. Mutation of the (AUU)3 motif abrogated the enhancement of EDEN-dependent deadenylation. These data indicate that the rate at which a specific maternal mRNA is deadenylated in Xenopus embryos is probably defined by a cross talk between multiple cis elements.
摘要
非洲爪蟾胚胎中母体mRNA的去腺苷酸化是一个序列特异性过程。一个在受精后将母体mRNA靶向去腺苷酸化的顺式元件是胚胎去腺苷酸化元件（EDEN）。该元件由U/R重复序列组成，被一种蛋白质EDEN-BP特异性结合。在本研究中，我们表明，含有EDEN的RNA的去腺苷酸化速率可以通过存在一个由三个AUU重复序列组成的额外顺式元件而增加。对于天然的EDEN（c-mos）和两个合成的EDEN，都观察到了这种效应。因此，由（AUU）3基序赋予的EDEN依赖性去腺苷酸化的增强不是由于与特定EDEN序列的相互作用。（AUU）3基序的突变消除了EDEN依赖性去腺苷酸化的增强。这些数据表明，非洲爪蟾胚胎中特定母体mRNA的去腺苷酸化速率可能由多个顺式元件之间的相互作用决定。
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本文引用的文献
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EDEN and EDEN-BP, a cis element and an associated factor that mediate sequence-specific mRNA deadenylation in Xenopus embryos.EDEN和EDEN-BP，一种顺式元件和一种相关因子，它们介导非洲爪蟾胚胎中序列特异性mRNA的去腺苷酸化。
EMBO J. 1998 Jan 2;17(1):278-87. doi: 10.1093/emboj/17.1.278.
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Postfertilization deadenylation of mRNAs in Xenopus laevis embryos is sufficient to cause their degradation at the blastula stage.非洲爪蟾胚胎中mRNA的受精后去腺苷酸化足以导致其在囊胚期降解。
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Further analysis of cytoplasmic polyadenylation in Xenopus embryos and identification of embryonic cytoplasmic polyadenylation element-binding proteins.非洲爪蟾胚胎中细胞质多聚腺苷酸化的进一步分析及胚胎细胞质多聚腺苷酸化元件结合蛋白的鉴定。
Mol Cell Biol. 1994 Dec;14(12):7867-75. doi: 10.1128/mcb.14.12.7867-7875.1994.
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