Uchinuno Y, Noda Y, Ishibashi K, Nagasue S, Shirakawa H, Nagano M, Ohe R
Fukuoka Chuo Livestock Hygiene Service Center, Japan.
J Vet Med Sci. 1998 Oct;60(10):1139-40. doi: 10.1292/jvms.60.1139.
A male fetus of gestation day 187 was aborted from a Holstein-Friesian cow in an epizootic of the Aino virus (AINOV) in September 1995. Neutralizing antibody titers against AINOV were 1:128, 1:16 and 1:64 in the dam serum, fetal ascites and cerebrospinal fluid, respectively. A 10% brain suspension of the aborted fetus was prepared immediately after autopsy, rinsed three times and sonicated before centrifugation. The supernatant was then inoculated into HmLu-1 cell cultures. A cytopathic effect was noted on post-inoculation day 7. The isolated virus was identified as the AINOV based on the physicochemical properties and cross neutralization test. This is the first report on the isolation of AINOV from an aborted bovine fetus.
1995年9月，在一次阿依努病毒（AINOV） epizootic中，一头荷斯坦-弗里生奶牛流产了一头妊娠187天的雄性胎儿。母畜血清、胎儿腹水和脑脊液中针对AINOV的中和抗体效价分别为1:128、1:16和1:64。流产胎儿尸检后立即制备10%脑悬液，冲洗三次并超声处理后离心。然后将上清液接种到HmLu-1细胞培养物中。接种后第7天观察到细胞病变效应。根据理化性质和交叉中和试验，分离出的病毒被鉴定为AINOV。这是关于从流产牛胎儿中分离出AINOV的首次报道。
