Giménez-Barcons M, Ibáñez A, Tajahuerce A, Sánchez-Tapias J M, Rodés J, Martínez M A, Saiz J C
Department of Medicine, Institut d'Investigacions Biomediques August Pi i Sunyer, Hospital Clinic, Universitat de Barcelona, Spain.
J Gen Virol. 1998 Nov;79 ( Pt 11):2623-9. doi: 10.1099/0022-1317-79-11-2623.
Comparative sequence analysis of different isolates of hepatitis G virus (HGV) has demonstrated significant intersubject genetic heterogeneity, but few data on intrasubject genetic evolution have been reported. To further investigate the genetic diversification of the HGV genome, 36 plasma samples from eleven patients chronically infected with HGV serially obtained 2-4 years apart were analysed. We determined the viral nucleotide sequence of the 5' non-coding (NC) and the NS3 regions by directly sequencing the RT-PCR amplified products obtained from the viral RNAs. Intrasubject sequence variation was found to be 1.3-2.4 x 10(-3) base substitutions per genome site per year within the 5' NC region and 1.3-9.4 x 10(-3) base substitutions per genome site per year within the NS3 region. Depending on the genomic region analysed (i.e. 5' NC or NS3 region), pairwise comparisons and phylogenetic reconstructions showed that intersubject genetic distances were 17.5- to 20.8-fold greater than intrasubject ones. Overall, the evolution rate of HGV in the regions analysed is not significantly different from that found in hepatitis C virus.
庚型肝炎病毒（HGV）不同分离株的比较序列分析已显示出显著的个体间基因异质性，但关于个体内基因进化的数据报道较少。为了进一步研究HGV基因组的基因多样化，我们分析了11例慢性感染HGV患者的36份血浆样本，这些样本是在相隔2 - 4年的时间里陆续采集的。我们通过对从病毒RNA中获得的RT-PCR扩增产物进行直接测序，确定了5'非编码（NC）区和NS3区的病毒核苷酸序列。在5' NC区内，个体内序列变异为每年每个基因组位点1.3 - 2.4×10⁻³个碱基替换，在NS3区内为每年每个基因组位点1.3 - 9.4×10⁻³个碱基替换。根据所分析的基因组区域（即5' NC区或NS3区），成对比较和系统发育重建显示个体间的基因距离比个体内的基因距离大17.5至20.8倍。总体而言，在所分析区域中HGV的进化速率与丙型肝炎病毒的进化速率没有显著差异。
