Viazov S, Riffelmann M, Khoudyakov Y, Fields H, Varenholz C, Roggendorf M
Ivanovsky Institute of Virology, Moscow, Russia.
J Gen Virol. 1997 Mar;78 ( Pt 3):577-81. doi: 10.1099/0022-1317-78-3-577.
Comparative sequence analysis of a 354 nt fragment of the NS5 region of hepatitis G virus (HGV) isolates was performed to assess two levels of HGV genomic variability: (1) heterogeneity of HGV within an infected individual, and (2) heterogeneity of different HGV isolates. Comparison of nucleotide sequences of DNA clones from two virus isolates demonstrated that in each infected individual HGV is represented by a population of virions with closely related but heterogeneous genomes (quasi-species). Phylogenetic analysis of nucleotide sequences of 42 isolates collected from 14 countries revealed less significant genome variability of HGV as compared to hepatitis C virus. Sequences of all HGV isolates fell into one group of distribution of evolutionary distances. On a phylogenetic tree all HGV sequences segregated into numerous branches. All sequences of isolates from Africa, South and South-East Asia, however, were clustered together and were separated from those of other isolates collected in Europe, North America and Central Asia.
对庚型肝炎病毒（HGV）分离株NS5区域的354个核苷酸片段进行了比较序列分析，以评估HGV基因组变异的两个层面：（1）感染个体内HGV的异质性，以及（2）不同HGV分离株的异质性。对来自两种病毒分离株的DNA克隆的核苷酸序列进行比较表明，在每个感染个体中，HGV由一群具有密切相关但异质基因组的病毒粒子（准种）代表。对从14个国家收集的42个分离株的核苷酸序列进行系统发育分析表明，与丙型肝炎病毒相比，HGV的基因组变异不太显著。所有HGV分离株的序列都属于进化距离分布的一组。在系统发育树上，所有HGV序列都分成许多分支。然而，来自非洲、南亚和东南亚的分离株的所有序列都聚集在一起，并与在欧洲、北美和中亚收集的其他分离株的序列分开。
