Ebert M P, Ademmer K, Müller-Ostermeyer F, Friess H, Büchler M W, Schubert W, Malfertheiner P
Department of Gastroenterology, Hepatology and Infectious Diseases, and Institute of Medical Neurobiology, University of Magdeburg, Germany.
Am J Gastroenterol. 1998 Nov;93(11):2141-7. doi: 10.1111/j.1572-0241.1998.00610.x.
Chronic pancreatitis is a painful chronic inflammatory disease of the exocrine pancreas that is associated with the replacement of functional parenchyma by extended fibrosis and with a massive infiltration of T lymphocytes. However, to date further characterization of infiltrating T cells in chronic pancreatitis has not been undertaken.
Using the novel method of multiepitope imaging with fluorochrome-tagged specific monoclonal antibodies, which allows the simultaneous localization and characterization of T cells in tissues, we analyzed the distribution and phenotypes of T cells infiltrating the pancreas in chronic pancreatitis.
The mean CD4:CD8 ratio in 10 cases of chronic pancreatitis was 2.4:1. In order of decreasing frequency, the following markers were observed: CD45RO, CD18, TCRgammadelta, and CD103. The lymphocytes, especially of the CD4+ subset, were found mainly in the fibrous stroma, but T cells were also observed periductally. A T-cell subset bearing the phenotype CD8+CD103+, analogous to intestinal intraepithelial lymphocytes, was found intracalating between the cells of the ductal epithelium.
Phenotyping of the T lymphocytes in chronic pancreatitis supports the concept of the involvement of cell-mediated cytotoxicity in the pathogenesis of this disease. In addition, intraepithelial lymphocytes were found interspersed between the ductal epithelial cells, pointing to a role of this T-cell subset as a first-line defense against deleterious epithelial events in chronic pancreatitis.
慢性胰腺炎是一种外分泌胰腺的疼痛性慢性炎症性疾病，与功能性实质被广泛纤维化替代以及T淋巴细胞大量浸润有关。然而，迄今为止尚未对慢性胰腺炎中浸润性T细胞进行进一步的特征描述。
我们使用了一种新型的多表位成像方法，即使用荧光染料标记的特异性单克隆抗体，该方法能够同时定位和鉴定组织中的T细胞，分析了慢性胰腺炎中浸润胰腺的T细胞的分布和表型。
10例慢性胰腺炎患者的平均CD4:CD8比值为2.4:1。按频率递减顺序观察到以下标志物：CD45RO、CD18、TCRγδ和CD103。淋巴细胞，尤其是CD4+亚群，主要存在于纤维基质中，但在导管周围也观察到T细胞。发现一种具有CD8+CD103+表型的T细胞亚群，类似于肠道上皮内淋巴细胞，穿插于导管上皮细胞之间。
慢性胰腺炎中T淋巴细胞的表型分析支持细胞介导的细胞毒性参与该疾病发病机制的概念。此外，发现上皮内淋巴细胞穿插于导管上皮细胞之间，表明该T细胞亚群在慢性胰腺炎中作为抵御有害上皮事件的一线防御发挥作用。
