Scheuerman TR, Camper AK, Hamilton MA
Center for Biofilm Engineering, Montana State University, 366 EPS Building, Bozeman, Montana, 59717-3980
J Colloid Interface Sci. 1998 Dec 1;208(1):23-33. doi: 10.1006/jcis.1998.5717.
The effect of substratum topography on bacterial surface colonization was studied using a chemically homogeneous silicon coupon. "Grooves" 10 µm deep and 10, 20, 30, and 40 µm wide were etched on the coupon perpendicular to the direction of flow. Flow (Re = 5.5) of a bacterial suspension (10(8) cells/ml) was directed through a parallel plate flow chamber inverted on a confocal microscope. Images were collected in real time to obtain rate and endpoint colonization data for each of three strains of bacteria: Pseudomonas aeruginosa and motile and nonmotile Pseudomonas fluorescens. A higher velocity experiment (Re = 16.6) and an abiotic control using hydrophilic, negatively charged microspheres were also performed. Using a colloidal deposition expression, the initial rates of attachment were compared. P. aeruginosa attached at a higher rate than P. fluorescens mot+ which attached at a higher rate than P. fluorescens mot-. For all bacteria the rate was independent of groove size and was greatest on the downstream edges of the grooves. Only the motile organisms were found in the bottoms of the grooves. A higher fluid velocity resulted in an increase in the initial rate of attachment. In contrast, there was no adhesion of the beads. Attachment of the bacteria appears to be predominated by transport from the bulk phase to the substratum. Copyright 1998 Academic Press.
使用化学性质均一的硅片研究了基质形貌对细菌表面定殖的影响。在硅片上垂直于流动方向蚀刻出深度为10 µm、宽度分别为10、20、30和40 µm的“凹槽”。将细菌悬浮液（10⁸ 个细胞/毫升）以一定流速（雷诺数Re = 5.5）引导通过倒置在共聚焦显微镜上的平行平板流动腔。实时采集图像以获取三种细菌菌株（铜绿假单胞菌、运动型荧光假单胞菌和非运动型荧光假单胞菌）各自的定殖速率和终点定殖数据。还进行了一个更高流速的实验（Re = 16.6）以及使用亲水性带负电荷微球的非生物对照实验。使用胶体沉积表达式比较了初始附着速率。铜绿假单胞菌的附着速率高于运动型荧光假单胞菌，运动型荧光假单胞菌的附着速率高于非运动型荧光假单胞菌。对于所有细菌，附着速率与凹槽尺寸无关，且在凹槽的下游边缘处最大。仅在凹槽底部发现了运动型生物体。更高的流体流速导致初始附着速率增加。相比之下，微球没有发生黏附。细菌的附着似乎主要由从本体相到基质的输运主导。版权所有1998年学术出版社。
