Hitoto T, Tsuruoka M, Hiruma Y, Matsui Y
Department of Physiology, Showa University School of Dentistry, Tokyo, Japan.
Neurochem Res. 1998 Dec;23(12):1461-5. doi: 10.1007/BF03177488.
We compared the noradrenaline (NA) level in the dorsal horn following electrical stimulation of A delta afferent nerve fibers in the peripheral nervous system between rats with bilateral lesions of the locus coeruleus (LC) and non-operated control rats by using a microdialysis technique combined with high performance liquid chromatography. Prior to A delta afferent fiber stimulation, the NA content in the dialysate did not differ between the LC-lesioned and the control rats. During A delta afferent fiber stimulation, in the LC-lesioned rats, the NA level did not change significantly compared to that before A delta afferent fiber stimulation, whereas the NA level increased significantly in the control rats. There was a significant difference in the NA levels during A delta afferent fiber stimulation between the two groups of rats. The result suggests that descending noradrenergic neurons from the LC is involved in the increase of the NA level in the spinal cord dorsal horn produced by A delta afferent fiber stimulation.
我们采用微透析技术结合高效液相色谱法，比较了蓝斑（LC）双侧损伤大鼠与未手术的对照大鼠在电刺激外周神经系统中的Aδ传入神经纤维后脊髓背角去甲肾上腺素（NA）水平的差异。在刺激Aδ传入纤维之前，LC损伤大鼠与对照大鼠透析液中的NA含量没有差异。在刺激Aδ传入纤维期间，LC损伤大鼠的NA水平与刺激Aδ传入纤维之前相比没有显著变化，而对照大鼠的NA水平显著升高。两组大鼠在刺激Aδ传入纤维期间的NA水平存在显著差异。结果表明，来自LC的下行去甲肾上腺素能神经元参与了Aδ传入纤维刺激引起的脊髓背角NA水平的升高。
