Yadav R P, Saxena R K, Gupta R, Davidson S
Department of Microbiology, University of Delhi, India.
Folia Microbiol (Praha). 1998;43(4):373-8. doi: 10.1007/BF02818576.
Forty each of aspergilli and penicillia were screened for extracellular lipase production on agar plates and in liquid medium containing olive oil as substrate. Twenty-nine aspergilli and twenty-six penicillia produced lipase. Out of these, 19 aspergilli and 22 penicillia showed activity both on Nile blue sulfate and glycerol tributyrate agar plates while only 10 aspergilli and 4 penicillia showed a positive response to glycerol tributyrate agar alone. The screening revealed 11 Aspergillus spp. and 15 Penicillium spp. as new lipase producers. Pig fat as an economic substrate for lipase production was also investigated.
在以橄榄油为底物的琼脂平板和液体培养基上，对40株曲霉菌和40株青霉菌进行了胞外脂肪酶产生情况的筛选。29株曲霉菌和26株青霉菌产生了脂肪酶。其中，19株曲霉菌和22株青霉菌在硫酸尼罗蓝和三丁酸甘油酯琼脂平板上均表现出活性，而只有10株曲霉菌和4株青霉菌仅对三丁酸甘油酯琼脂平板呈阳性反应。筛选出11个曲霉属物种和15个青霉属物种为新的脂肪酶产生菌。还研究了猪脂肪作为脂肪酶生产的经济底物的情况。
