F420H2:NADP oxidoreductase from Methanobacterium thermoautotrophicum: identification of the encoding gene via functional overexpression in Escherichia coli.
作者信息
Berk H, Thauer R K
机构信息
Max-Planck-Institut für terrestrische Mikrobiologie and Laboratorium für Mikrobiologie, Fachbereich Biologie der Philipps-Universität Marburg, Germany.
出版信息
FEBS Lett. 1998 Oct 30;438(1-2):124-6. doi: 10.1016/s0014-5793(98)01288-5.
PMID:9821972
Abstract
F420H2:NADP oxidoreductase is found in methanogenic, sulfate-reducing and halophilic archaea and also in some bacteria. The putative gene encoding the enzyme was cloned from Methanobacterium thermoautotrophicum (strain Marburg) and heterologously expressed in Escherichia coli. The overproduced active enzyme was purified, characterized and crystallized.
摘要
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