Santolaya-Forgas J, Duval J, Prespin C, Vengalil S, Kushwaha A, Wilson L, Fortman J
Department of Obstetrics and Gynecology and Biological Resources Laboratory, University of Illinois at Chicago, IL 60612, USA.
Am J Obstet Gynecol. 1998 Nov;179(5):1124-7. doi: 10.1016/s0002-9378(98)70117-6.
Access to extracoelomic fluid offers the opportunity to assess and potentially treat genetic disorders early in pregnancy. We have been using the pregnant baboon as a model to develop techniques and evaluate the feasibility of sampling extracoelomic fluid. The aim of this study was to determine the osmolality, oncotic pressures, and electrolyte composition of the baboon's extracoelomic fluid between days 39 and 41 of gestation and to compare them with those of maternal blood.
The optimal time to perform the coelocentesis procedure was determined in 14 timed pregnant baboons. Six coelocenteses were then performed in aseptic conditions, under continuous transvaginal ultrasonographic guidance and avoiding the amniotic or yolk sacs. Between 3 and 5 mL extracoelomic fluid was aspirated from each baboon with a 10-mL syringe. Only 1 attempt at sampling was performed for each of the 6 animals. Pregnancies were tracked by transabdominal ultrasonographic evaluations on postprocedure day 3 and then weekly until day 140 of pregnancy. Oncotic pressures and biochemical measurements were determined with 1 mL extracoelomic fluid and 1 mL heparinized maternal venous blood.
Data analysis suggests that maternal blood and extracoelomic fluid have similar osmolalities and concentrations of electrolytes but different colloid osmotic pressures (P <.001).
This nonhuman primate model can be used to gain some insight into the physiologic changes in the composition of the extracoelomic fluid and to evaluate the safety of the coelocentesis procedure. The data suggest that the chorion laeve behaves as a semipermeable membrane at 40 days' gestation.
获取体腔外液为在妊娠早期评估并可能治疗遗传性疾病提供了机会。我们一直使用怀孕的狒狒作为模型来开发技术并评估采集体腔外液的可行性。本研究的目的是确定妊娠39至41天狒狒体腔外液的渗透压、胶体渗透压和电解质组成，并将其与母体血液的相应指标进行比较。
在14只定时怀孕的狒狒中确定进行体腔穿刺术的最佳时间。然后在无菌条件下，在持续经阴道超声引导下，避免穿刺羊膜或卵黄囊，进行6次体腔穿刺。用10毫升注射器从每只狒狒中抽取3至5毫升体腔外液。对6只动物中的每只仅进行1次采样尝试。术后第3天通过经腹超声评估跟踪妊娠情况，然后每周评估一次，直至妊娠第140天。用1毫升体腔外液和1毫升肝素化母体静脉血测定胶体渗透压和生化指标。
数据分析表明，母体血液和体腔外液具有相似的渗透压和电解质浓度，但胶体渗透压不同（P<.001）。
这个非人灵长类动物模型可用于深入了解体腔外液成分的生理变化，并评估体腔穿刺术的安全性。数据表明，在妊娠40天时，平滑绒毛膜表现为半透膜。
