Sivan E, Whittaker P G, Sinha D, Homko C J, Lin M, Reece E A, Boden G
Department of Obstetrics and Gynecology, Division of Endocrinology, Diabetes, Metabolism, General Clinical Research Center, Temple University School of Medicine, Philadelphia, PA 19140, USA.
Am J Obstet Gynecol. 1998 Nov;179(5):1128-32. doi: 10.1016/s0002-9378(98)70118-8.
The aims of the study were (1) to examine the relationship between leptin and placental hormones by measuring serial changes in serum levels of leptin during and after pregnancy and (2) to study the effects of several gestational hormones on leptin release from fully differentiated 3T3-L1 adipocyte cell cultures.
Serum levels of leptin were measured throughout pregnancy and at 3 months post partum in 29 healthy women and were also measured in 18 healthy women at delivery by cesarean section and on postpartum day 3. In addition, 3T3-L1 mouse adipocytes were incubated for 24 hours in media containing various reproductive hormones and leptin production was measured.
Serum leptin levels increased significantly (8.4 +/- 0.9 vs 13.5 +/- 1.5 ng/mL; P <.001) between the first 2 trimesters of pregnancy but not between the second and third trimesters. These changes in leptin did not correlate significantly with changes in body mass index. Leptin levels dropped significantly during the immediate postpartum period, from 34.1 +/- 4.9 at cesarean delivery to 7.3 +/- 1.4 ng/mL on postpartum day 3 (P <.001). Fasting insulin level did not correlate significantly with leptin level during pregnancy but did so during the postpartum period (r = 0.60; P <.05). Leptin secretion from 3T3-L1 adipocytes was increased significantly when cells were cultured with human chorionic gonadotropin (150%, P <.01) and also when they were cultured with estrogen (120%, P <.03).
The data suggest that leptin production by adipose tissue is stimulated by several hormones of pregnancy, which may contribute to the increased leptin levels observed during gestation.
本研究的目的是：（1）通过测量孕期及产后血清瘦素水平的系列变化，研究瘦素与胎盘激素之间的关系；（2）研究几种妊娠激素对完全分化的3T3-L1脂肪细胞培养物中瘦素释放的影响。
对29名健康女性在整个孕期及产后3个月测量血清瘦素水平，并对18名健康女性在剖宫产分娩时及产后第3天测量血清瘦素水平。此外，将3T3-L1小鼠脂肪细胞在含有各种生殖激素的培养基中孵育24小时，并测量瘦素的产生。
孕期前两个阶段之间血清瘦素水平显著升高（8.4±0.9 vs 13.5±1.5 ng/mL；P<.001），但在第二和第三孕期之间没有显著变化。瘦素的这些变化与体重指数的变化没有显著相关性。产后即刻瘦素水平显著下降，从剖宫产时的34.1±4.9降至产后第3天的7.3±1.4 ng/mL（P<.001）。孕期空腹胰岛素水平与瘦素水平没有显著相关性，但在产后阶段有显著相关性（r = 0.60；P<.05）。当3T3-L1脂肪细胞与人绒毛膜促性腺激素一起培养时，瘦素分泌显著增加（150%，P<.01），与雌激素一起培养时也显著增加（120%，P<.03）。
数据表明，妊娠的几种激素刺激脂肪组织产生瘦素，这可能是孕期观察到瘦素水平升高的原因。
