Rich T, Johnson F B
Department of Microbiology, Brigham Young University, Provo, UT 84602, USA.
Diagn Microbiol Infect Dis. 1998 Oct;32(2):81-4. doi: 10.1016/s0732-8893(98)00071-6.
Six cell lines were assessed in the suspension-infection (SI) test for suitability for the rapid culture of herpes simplex virus (HSV). For the SI test, the specimen was combined in growth medium with trypsinized, suspended culture cells before allowing the cells to settle into a monolayer growth pattern. The cells tested in the SI assay were MV1-Lu, vero C1008, BSC-1, RD, and MRC-9 cells. Fifty clinical specimens composed of 7 HSV-1-positive samples, 9 HSV-2-positive samples, 12 positive for HSV (not typed), and 22 HSV-negative samples were tested. For the detection of HSV in clinical specimens, MV1Lu cells were most sensitive and demonstrated large, darkly stained foci of virus infection.
在悬浮感染（SI）试验中评估了六种细胞系对单纯疱疹病毒（HSV）快速培养的适用性。对于SI试验，将标本与胰蛋白酶处理后的悬浮培养细胞在生长培养基中混合，然后让细胞形成单层生长模式。在SI试验中测试的细胞是MV1-Lu、非洲绿猴肾细胞系C1008、非洲绿猴肾细胞系BSC-1、人横纹肌肉瘤细胞系RD和人胚肺成纤维细胞系MRC-9。对50份临床标本进行了检测，其中包括7份HSV-1阳性样本、9份HSV-2阳性样本、12份HSV阳性（未分型）样本和22份HSV阴性样本。为了检测临床标本中的HSV，MV1Lu细胞最为敏感，并且显示出大量深色染色的病毒感染病灶。
