一种用于检测单纯疱疹病毒的、可替代空斑试验法的快速培养方法。
A rapid culture alternative to the shell-vial method for the detection of herpes simplex virus.
作者信息
Johnson F B, Visick E M
机构信息
Department of Microbiology, Brigham Young University, Provo, UT 84602.
出版信息
Diagn Microbiol Infect Dis. 1992 Nov-Dec;15(8):673-8. doi: 10.1016/0732-8893(92)90070-a.
The rapid test for detection of herpes simplex virus (HSV) in clinical specimens based on infection of cells in suspension (SI test) was compared to the shell-vial culture (SVC) method and conventional culture. Mink lung cells were used throughout the study. Detection of HSV was not significantly different whether using SI or SVC. The sensitivity of SI in detecting HSV, when compared with conventional culture, was 93.0% using 0.1 ml inocula and 98.3% using 0.5 ml inocula. The time to obtain a final result with both SI and SVC was 1 day compared with 1-7 days by conventional culture. The SI method detected both HSV type-1 and HSV type-2 clinical isolates. The SI technique is a simple method for the rapid detection of HSV and can yield diagnostic results with a minimum of technical manipulations.
基于悬浮细胞感染法（SI试验）对临床标本中单纯疱疹病毒（HSV）进行快速检测，并与空斑小瓶培养法（SVC）和传统培养法进行比较。整个研究过程中均使用貂肺细胞。无论是使用SI试验还是SVC法，HSV的检测结果均无显著差异。与传统培养法相比，使用0.1 ml接种物时，SI试验检测HSV的灵敏度为93.0%，使用0.5 ml接种物时为98.3%。SI试验和SVC法获得最终结果的时间均为1天，而传统培养法为1 - 7天。SI试验方法可检测HSV - 1型和HSV - 2型临床分离株。SI技术是一种快速检测HSV的简单方法，只需最少的技术操作就能得出诊断结果。
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